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Literature summary for 6.3.4.16 extracted from

  • Lof, C.; Cohen, M.; Vermeulen, L.P.; van Roermund, C.W.T.; Wanders, R.J.A.; Meijer, A.J.
    Properties of carbamoyl-phosphate synthetase (ammonia) in rat-liver mitochondria made permeable with toluene (1983), Eur. J. Biochem., 135, 251-258.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetylglutamate activates Rattus norvegicus
acetylglutamate binding of acetylglutamate to carbamoylphosphate synthetase requires the presence of both Mg2+ and MgATP2-. The binding is inhibited by Ca2+ and N-carbamoylglutamate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ binding of acetylglutamate to carbamoylphosphate synthetase is inhibited Rattus norvegicus
Carbamoyl phosphate 50% inhibition at 13 mM Rattus norvegicus
N-carbamoylglutamate binding of acetylglutamate to carbamoylphosphate synthetase is inhibited Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
NH3
-
Rattus norvegicus
1.2
-
ATP
-
Rattus norvegicus
2
-
NH4+
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + NH3 + CO2 + H2O Rattus norvegicus first enzyme involved in the biosynthesis of urea in the liver ureotelic animals ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + NH3 + CO2 + H2O first enzyme involved in the biosynthesis of urea in the liver ureotelic animals Rattus norvegicus ?
-
?
2 ATP + NH4+ + HCO3- ATP in form of MgATP2- Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
?
ATP + NH4+ + CO2 + H2O
-
Rattus norvegicus ADP + phosphate + carbamoylphosphate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
13
-
Carbamoyl phosphate
-
Rattus norvegicus