Application | Comment | Organism |
---|---|---|
analysis | systematic mutagenesis method to find critical residues for certain physico-chemical properties of a protein. The method is a shorter alternative to random mutagenesis, saturation mutagenesis or directed evolution to find multiple amino acids critical for certain properties of proteins | Trypanosoma brucei brucei |
analysis | systematic mutagenesis method to find critical residues for certain physico-chemical properties of a protein. The method is a shorter alternative to random mutagenesis, saturation mutagenesis or directed evolution to find multiple amino acids critical for certain properties of proteins | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57 | Trypanosoma brucei brucei |
additional information | construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57 | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma brucei brucei | |
0.45 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma cruzi | 20015 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | P04789 | - |
- |
Trypanosoma cruzi | P52270 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei brucei | dihydroxyacetone 3-phosphate | - |
? | |
D-glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | dihydroxyacetone 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5167 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma cruzi | |
6000 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma brucei brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11500 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma cruzi | |
13600 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH not specified in the publication | Trypanosoma brucei brucei |