Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
S211A | mutation eliminates intraloop hydrogen bonds to the side-chain hydroxyl, 60fold decrease in kcat/Km | Saccharomyces cerevisiae |
S211G | mutation eliminates intraloop hydrogen bonds to the side-chain hydroxyl, leading to small changes in the kinetic parameters | Saccharomyces cerevisiae |
Y208A | main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy | Saccharomyces cerevisiae |
Y208F | mutation eliminates the intraloop hydrogen bond between the hydroxyl group of Y208 and the amide nitrogen of A176. Enzyme activity is reduced by ca. 50fold compared to the Y208A and Y208S mutants | Saccharomyces cerevisiae |
Y208S | main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy | Saccharomyces cerevisiae |
Y208T | main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy | Saccharomyces cerevisiae |
Y208T/S211G | tenfold decrease in kcat/Km | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
1 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
1.7 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
1.8 | - |
D-glyceraldehyde 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2.4 | - |
D-glyceraldehyde 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2.9 | - |
D-glyceraldehyde 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
3.2 | - |
dihydroxyacetone 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
3.4 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
3.9 | - |
D-glyceraldehyde 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
4 | - |
dihydroxyacetone 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
11 | - |
dihydroxyacetone 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
12 | - |
D-glyceraldehyde 3-phosphate | mutant S211A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
17 | - |
dihydroxyacetone 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
17 | - |
dihydroxyacetone 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
25 | - |
dihydroxyacetone 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00942 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | dihydroxyacetone 3-phosphate | - |
? | |
dihydroxyacetone 3-phosphate | - |
Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
dihydroxyacetone 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
13 | - |
D-glyceraldehyde 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
135 | - |
dihydroxyacetone 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
210 | - |
dihydroxyacetone 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
250 | - |
dihydroxyacetone 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
520 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
580 | - |
dihydroxyacetone 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
740 | - |
D-glyceraldehyde 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
810 | - |
dihydroxyacetone 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
860 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
940 | - |
D-glyceraldehyde 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2800 | - |
D-glyceraldehyde 3-phosphate | mutant S211A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
3700 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
7500 | - |
D-glyceraldehyde 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
8900 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 5 | arsenate | above 25 mM, mutant S211A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2 | 5 | arsenate | above 25 mM, mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2 | 5 | arsenate | above 25 mM, mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2 | 5 | arsenate | above 25 mM, mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
2 | 5 | arsenate | above 25 mM, mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
6.5 | - |
arsenate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
14 | - |
arsenate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
18 | - |
arsenate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
dihydroxyacetone 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
5.4 | - |
D-glyceraldehyde 3-phosphate | mutant Y208F, pH 7.5, 25°C | Saccharomyces cerevisiae | |
10 | - |
dihydroxyacetone 3-phosphate | mutant S211A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
10 | - |
dihydroxyacetone 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
12 | - |
dihydroxyacetone 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
34 | - |
dihydroxyacetone 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
53 | - |
dihydroxyacetone 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
230 | - |
D-glyceraldehyde 3-phosphate | mutant S211A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
240 | - |
D-glyceraldehyde 3-phosphate | mutant Y208S, pH 7.5, 25°C | Saccharomyces cerevisiae | |
250 | - |
dihydroxyacetone 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
260 | - |
D-glyceraldehyde 3-phosphate | mutant Y208A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
510 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
730 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T/S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
1100 | - |
D-glyceraldehyde 3-phosphate | mutant Y208T, pH 7.5, 25°C | Saccharomyces cerevisiae | |
4200 | - |
D-glyceraldehyde 3-phosphate | mutant S211G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
8900 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |