Literature summary for 5.3.1.1 extracted from

Bandyopadhyay, D.; Murthy, M.R.; Balaram, H.; Balaram, P.
Probing the role of highly conserved residues in triosephosphate isomerase - analysis of site specific mutants at positions 64 and 75 in the Plasmodial enzyme (2015), FEBS J., 282, 3863-3882 .

Search for more literature for 5.3.1.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of wild-type and mutants at residues T75 and Q64	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
Q64E	mutation weakens the dimeric structure	Plasmodium falciparum
Q64N	mutation weakens the dimeric structure	Plasmodium falciparum
T75C	activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C	Plasmodium falciparum
T75N	4fold drop in activity compared to wild-type. Decrease in stability between 35°C and 45°C	Plasmodium falciparum
T75S	activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C	Plasmodium falciparum
T75V	activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.62	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.6, 23°C	Plasmodium falciparum
0.8	-	D-glyceraldehyde 3-phosphate	mutant T75S, pH 7.6, 23°C	Plasmodium falciparum
0.86	-	D-glyceraldehyde 3-phosphate	mutant T75N, pH 7.6, 23°C	Plasmodium falciparum
0.91	-	D-glyceraldehyde 3-phosphate	mutant T75V, pH 7.6, 23°C	Plasmodium falciparum
1.15	-	D-glyceraldehyde 3-phosphate	mutant T75C, pH 7.6, 23°C	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q07412	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
63.3	-	mutant Q64E, pH 7.6, 23°C	Plasmodium falciparum
134	-	mutant Q64N, pH 7.6, 23°C	Plasmodium falciparum
679	-	wild-type, pH 7.6, 23°C	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Plasmodium falciparum	dihydroxyacetone 3-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.013	-	D-glyceraldehyde 3-phosphate	mutant T75N, pH 7.6, 23°C	Plasmodium falciparum
0.034	-	D-glyceraldehyde 3-phosphate	mutant T75C, pH 7.6, 23°C	Plasmodium falciparum
0.038	-	D-glyceraldehyde 3-phosphate	mutant T75S, pH 7.6, 23°C	Plasmodium falciparum
0.038	-	D-glyceraldehyde 3-phosphate	mutant T75V, pH 7.6, 23°C	Plasmodium falciparum
0.04	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.6, 23°C	Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
151	-	D-glyceraldehyde 3-phosphate	mutant T75N, pH 7.6, 23°C	Plasmodium falciparum
296	-	D-glyceraldehyde 3-phosphate	mutant T75C, pH 7.6, 23°C	Plasmodium falciparum
418	-	D-glyceraldehyde 3-phosphate	mutant T75V, pH 7.6, 23°C	Plasmodium falciparum
475	-	D-glyceraldehyde 3-phosphate	mutant T75S, pH 7.6, 23°C	Plasmodium falciparum
645	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.6, 23°C	Plasmodium falciparum

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Release 2023.1 (January 2023)