Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylmethane thiosulfonate | induces 75% enzyme inactivation within 15 min at 0.25 mM concentration | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.328 | - |
D-glyceraldehyde 3-phosphate | in 50 mM Tris, pH 7.6, at 25°C | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27600 | - |
2 * 27600, estimated from SDS-PAGE | Leishmania donovani |
54000 | - |
gel filtration | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | Leishmania donovani | - |
dihydroxyacetone phosphate | - |
? | |
D-glyceraldehyde 3-phosphate | Leishmania donovani MHOM/IN/80/Dd8 | - |
dihydroxyacetone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Leishmania donovani MHOM/IN/80/Dd8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Q-Sepharose column chromatography and Sephadex G-100 gel filtration | Leishmania donovani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.01 | - |
total cell lysate, in 50 mM Tris, pH 7.6, at 25°C | Leishmania donovani |
8.76 | - |
after 8.7fold purification, in 50 mM Tris, pH 7.6, at 25°C | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Leishmania donovani | dihydroxyacetone phosphate | - |
? | |
D-glyceraldehyde 3-phosphate | - |
Leishmania donovani MHOM/IN/80/Dd8 | dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27600, estimated from SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Leishmania donovani |
Triosephosphate isomerase | - |
Leishmania donovani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | the recombinant enzyme is stable at 25°C for 30 min. The recombinant enzyme retains 92.7%, 40% and 13% activity when preincubated at 37°C, 42°C and 60°C for 30 min, respectively | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9 | - |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the recombinant protein shows more than 90% activity over the pH range 7.2-9.0 and less than 70% activity below pH 7.0 or pH above pH 10.0 | Leishmania donovani |