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Literature summary for 5.3.1.1 extracted from

  • Zhai, X.; Amyes, T.L.; Wierenga, R.K.; Loria, J.P.; Richard, J.P.
    Structural mutations that probe the interactions between the catalytic and dianion activation sites of triosephosphate isomerase (2013), Biochemistry, 52, 5928-5940.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.1

Activating Compound

Activating Compound Comment Organism Structure
phosphite
-
 Gallus gallus
phosphite
-
 Trypanosoma brucei brucei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 pLys S cells Trypanosoma brucei brucei
expressed in Escherichia coli FB215471(DE3) cells Gallus gallus

Protein Variants

Protein Variants Comment Organism
L7RM the mutant exhibits a 200fold decrease in kcat/Km for isomerization of D-glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The mutant exhibits a 25fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme. The mutation has little effect on the observed and intrinsic phosphodianion binding energy and only a modest effect on phosphite dianion activation of the enzyme Gallus gallus
P168A the mutation results in 50fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
2-Phosphoglycolate competitive inhibition Gallus gallus
2-Phosphoglycolate competitive inhibition Trypanosoma brucei brucei
arsenate competitive inhibition Gallus gallus
arsenate competitive inhibition Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
0.25
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
0.27
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
0.29
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
0.49
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
0.59
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
0.7
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
4
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate Gallus gallus
-
 dihydroxyacetone phosphate
-
 r
D-glyceraldehyde 3-phosphate Trypanosoma brucei brucei
-
 dihydroxyacetone phosphate
-
 r
dihydroxyacetone phosphate Gallus gallus
-
 D-glyceraldehyde 3-phosphate
-
 r
dihydroxyacetone phosphate Trypanosoma brucei brucei
-
 D-glyceraldehyde 3-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Trypanosoma brucei brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM Sepharose column chromatography Trypanosoma brucei brucei
DEAE-Sepharose column chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
 Gallus gallus dihydroxyacetone phosphate
-
 r
D-glyceraldehyde 3-phosphate
-
 Trypanosoma brucei brucei dihydroxyacetone phosphate
-
 r
dihydroxyacetone phosphate
-
 Gallus gallus D-glyceraldehyde 3-phosphate
-
 r
dihydroxyacetone phosphate
-
 Trypanosoma brucei brucei D-glyceraldehyde 3-phosphate
-
 r
glycolaldehyde
-
 Gallus gallus ?
-
 ?
glycolaldehyde
-
 Trypanosoma brucei brucei ?
-
 ?

Synonyms

Synonyms Comment Organism
TIM
-
 Gallus gallus
TIM
-
 Trypanosoma brucei brucei
Triosephosphate isomerase
-
 Gallus gallus
Triosephosphate isomerase
-
 Trypanosoma brucei brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
8
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
16
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
24
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
300
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
340
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
2100
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
3200
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
 2-Phosphoglycolate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
0.055
-
 2-Phosphoglycolate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
0.14
-
 2-Phosphoglycolate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
2.3
-
 2-Phosphoglycolate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
3.8
-
 arsenate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
4.6
-
 arsenate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
9.6
-
 arsenate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
10
-
 arsenate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
13
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
59
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
260
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
430
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
580
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
8400
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
11000
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus