Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Rhipicephalus microplus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES-Na, pH 7.5, and 1.4 M trisodium citrate dihydrate | Rhipicephalus microplus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoic acid) | about 50% inhibition at about 0.05 mM | Rhipicephalus microplus | |
methyl methanethiosulfonate | about 50% inhibition at about 0.05 mM | Rhipicephalus microplus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
D-glyceraldehyde 3-phosphate | in 100 mM triethanolamine, pH 7.4, at 25°C | Rhipicephalus microplus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Rhipicephalus microplus |
27188 | - |
2 * 27188, calculated from amino acid sequence | Rhipicephalus microplus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhipicephalus microplus | A8B3A8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Mono-Q column column chromatography | Rhipicephalus microplus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Rhipicephalus microplus | - |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, 40 days, no loss of activity | Rhipicephalus microplus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Rhipicephalus microplus | dihydroxyacetone phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27000, SDS-PAGE | Rhipicephalus microplus |
homodimer | 2 * 27188, calculated from amino acid sequence | Rhipicephalus microplus |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Rhipicephalus microplus |
Triosephosphate isomerase | - |
Rhipicephalus microplus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhipicephalus microplus | calculated from amino acid sequence | - |
7.7 |