Cloned (Comment) | Organism |
---|---|
mutant enzymes E97Q and E97D are expressed in Escherichia coli AA200 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
E97Q and E97D mutant enzymes, hanging drop vapor diffusion method | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
E165A | the mutant shows an approximately 9000fold drop in activity | Plasmodium falciparum |
E97D | the mutant shows a 100fold reduction in kca | Plasmodium falciparum |
E97Q | the mutant shows a 4000fold reduction in kca | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
0.5 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
1.2 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q07412 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Plasmodium falciparum | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Plasmodium falciparum |
Triose phosphate isomerase | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
4.2 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
4300 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
84 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum | |
12200 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C | Plasmodium falciparum |