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Literature summary for 5.3.1.1 extracted from

  • Samanta, M.; Murthy, M.R.; Balaram, H.; Balaram, P.
    Revisiting the mechanism of the triosephosphate isomerase reaction: the role of the fully conserved glutamic acid 97 residue (2011), ChemBioChem, 12, 1886-1896.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes E97Q and E97D are expressed in Escherichia coli AA200 cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
E97Q and E97D mutant enzymes, hanging drop vapor diffusion method Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
E165A the mutant shows an approximately 9000fold drop in activity Plasmodium falciparum
E97D the mutant shows a 100fold reduction in kca Plasmodium falciparum
E97Q the mutant shows a 4000fold reduction in kca Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
D-glyceraldehyde 3-phosphate wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
0.5
-
D-glyceraldehyde 3-phosphate mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
1.2
-
D-glyceraldehyde 3-phosphate mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q07412
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate
-
Plasmodium falciparum Glycerone phosphate
-
?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Plasmodium falciparum

Synonyms

Synonyms Comment Organism
TIM
-
Plasmodium falciparum
Triose phosphate isomerase
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
D-glyceraldehyde 3-phosphate mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
4.2
-
D-glyceraldehyde 3-phosphate mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
4300
-
D-glyceraldehyde 3-phosphate wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
D-glyceraldehyde 3-phosphate mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
84
-
D-glyceraldehyde 3-phosphate mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum
12200
-
D-glyceraldehyde 3-phosphate wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C Plasmodium falciparum