Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.1 extracted from

  • Malabanan, M.M.; Go, M.K.; Amyes, T.L.; Richard, J.P.
    Wildtype and engineered monomeric triosephosphate isomerase from Trypanosoma brucei: partitioning of reaction intermediates in D2O and activation by phosphite Dianion (2011), Biochemistry, 50, 5767-5779.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphite
-
Trypanosoma brucei brucei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 pLys S cells Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information the monomeric form of TIM does not catalyze any reactions of D-glyceraldehyde 3-phosphate at the enzyme active site, either in the absence or in the presence of phosphite dianion Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
D-glyceraldehyde 3-phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei
0.7
-
dihydroxyacetone phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and CM-Sepharose column chromatography Trypanosoma brucei brucei

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Trypanosoma brucei brucei dihydroxyacetone phosphate
-
r

Synonyms

Synonyms Comment Organism
TIM
-
Trypanosoma brucei brucei
Triosephosphate isomerase
-
Trypanosoma brucei brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
300
-
dihydroxyacetone phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei
2100
-
D-glyceraldehyde 3-phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
430
-
dihydroxyacetone phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei
8400
-
D-glyceraldehyde 3-phosphate wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C Trypanosoma brucei brucei