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Literature summary for 5.3.1.1 extracted from

  • Alahuhta, M.; Wierenga, R.K.
    Atomic resolution crystallography of a complex of triosephosphate isomerase with a reaction-intermediate analog: new insight in the proton transfer reaction mechanism (2010), Proteins, 78, 1878-1888.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme mutant E65Q in complex with reaction-intermediate analogue phosphoglycolohydroxamate, hanging drop method, TIM-PGH crystals are grown at room temperature, 0.004 ml of protein solution with 11 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl, 10 mM PGH, 1 mM DTT, 1 mM EDTA, and 1 mM NaN3, are mixed with 0.004 ml of well solution containing 0.1M acetate, pH 5.0, 24% PEG6000, 1 mM DTT, 1 mM EDTA, and 1 mM NaN3, X-ray diffraction structure determination and analysis at 0.82 A resolution Leishmania mexicana

Protein Variants

Protein Variants Comment Organism
E65Q the variant of Leishmania mexicana TIM has a much enhanced stability but its catalytic properties are the same as wild-type leishmanial TIM Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
Phosphoglycolohydroxamate the reaction-intermediate analogue binds to the active site with two hydrogen-bonding interactions between PGH and the Glu167 side-chain oxygen atoms Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Glyceraldehyde 3-phosphate Leishmania mexicana the reaction is essential in vivo Glycerone phosphate
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r

Organism

Organism UniProt Comment Textmining
Leishmania mexicana P48499
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-

Reaction

Reaction Comment Organism Reaction ID
D-Glyceraldehyde 3-phosphate = glycerone phosphate reaction mechanism via enediolate intermediate through proton abstraction by the catalytic base Glu167, overview Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate the reaction is essential in vivo Leishmania mexicana Glycerone phosphate
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r
D-Glyceraldehyde 3-phosphate triosephosphate isomerase is an enolizing enzyme, which catalyses the interconversion of dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate Leishmania mexicana Glycerone phosphate
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r
additional information the proton transfer reaction from PGH to the Glu167 side chain, when PGH becomes sequestered in the active site, active site modeling, overview Leishmania mexicana ?
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?

Subunits

Subunits Comment Organism
dimer structure comparisons, overview Leishmania mexicana

Synonyms

Synonyms Comment Organism
TIM
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Leishmania mexicana
Triosephosphate isomerase
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Leishmania mexicana

General Information

General Information Comment Organism
metabolism the dimeric enzyme functions in the glycolytic pathway. In the glycolysis, the catalyzed interconversion reaction is important in the thermodynamically uphill direction of the synthesis of D-glyceraldehyde 3-phosphate Leishmania mexicana