Cloned (Comment) | Organism |
---|---|
expression of the wild-type enzyme in TIM-deficient Escherichia coli strain DF502 | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Gallus gallus | - |
Glycerone phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme from TIM-deficient Escherichia coli strain DF502 by anion exchange chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
quantitative TIM-catalyzed isomerization of D-glyceraldehyde 3-phosphate to form glycerone phosphate is coupled to the oxidation of NADH using glycerol 3-phosphate dehydrogenase | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Gallus gallus | Glycerone phosphate | - |
r | |
D-Glyceraldehyde 3-phosphate | stereospecific reaction via an enzyme-bound enediol(ate) intermediate, proton transfer from D-glyceraldehyde 3-phosphate to the carboxylate side chain of TIM Glu165, irreversible labeling with deuterium , overview | Gallus gallus | Glycerone phosphate | - |
r | |
glycolaldehyde | TIM-catalyzed reactions of glycolaldehyde are activated by phosphite dianion, kinetics, overview | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Gallus gallus |
Triosephosphate isomerase | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Gallus gallus |