Literature summary for 5.3.1.1 extracted from

Cabrera, N.; Hernandez-Alcantara, G.; Mendoza-Hernandez, G.; Gomez-Puyou, A.; Perez-Montfort, R.
Key residues of loop 3 in the interaction with the interface residue at position 14 in triosephosphate isomerase from Trypanosoma brucei (2008), Biochemistry, 47, 3499-3506.

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Protein Variants

Protein Variants	Comment	Organism
C14S	wild-type-like enzyme that is resistant to the action of sulfhydryl reagents methylmethane thiosulfonate and 5,5-dithiobis(2-nitrobenzoate)	Trypanosoma brucei
C14S/A69C	similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin	Trypanosoma brucei
C14S/A73C	greatly reduced kcat value. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin	Trypanosoma brucei
C14S/S71C	similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin	Trypanosoma brucei
C14S/S79C	similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	D-glyceraldehyde 3-phosphate	mutant C14S/A73C, 25°C, pH 7.4	Trypanosoma brucei
0.35	-	D-glyceraldehyde 3-phosphate	mutant C14S/S71C, 25°C, pH 7.4	Trypanosoma brucei
0.38	-	D-glyceraldehyde 3-phosphate	wild-type, 25°C, pH 7.4	Trypanosoma brucei
0.48	-	D-glyceraldehyde 3-phosphate	mutant C14S/S79C, 25°C, pH 7.4	Trypanosoma brucei
0.5	-	D-glyceraldehyde 3-phosphate	mutant C14S, 25°C, pH 7.4	Trypanosoma brucei
0.75	-	D-glyceraldehyde 3-phosphate	mutant C14S/A69C, 25°C, pH 7.4	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Trypanosoma brucei	dihydroxyacetone phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
483	-	D-glyceraldehyde 3-phosphate	mutant C14S/A73C, 25°C, pH 7.4	Trypanosoma brucei
2600	-	D-glyceraldehyde 3-phosphate	mutant C14S/A69C, 25°C, pH 7.4	Trypanosoma brucei
2967	-	D-glyceraldehyde 3-phosphate	mutant C14S/S79C, 25°C, pH 7.4	Trypanosoma brucei
3200	-	D-glyceraldehyde 3-phosphate	mutant C14S/S71C, 25°C, pH 7.4	Trypanosoma brucei
4183	-	D-glyceraldehyde 3-phosphate	wild-type, 25°C, pH 7.4	Trypanosoma brucei
5133	-	D-glyceraldehyde 3-phosphate	mutant C14S, 25°C, pH 7.4	Trypanosoma brucei

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Release 2023.1 (January 2023)