Literature summary for 5.3.1.1 extracted from

Hernandez-Alcantara, G.; Garza-Ramos, G.; Hernandez, G.M.; Gomez-Puyou, A.; Perez-Montfort, R.
Catalysis and stability of triosephosphate isomerase from Trypanosoma brucei with different residues at position 14 of the dimer interface. Characterization of a catalytically competent monomeric enzyme (2002), Biochemistry, 41, 4230-4238.

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Protein Variants

Protein Variants	Comment	Organism
C14A	the KM-value for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 90% of the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is comparable to the value for the wild-type enzyme	Trypanosoma brucei
C14F	the Ki-value for 3-phosphoglycolate is nearly 3times higher than the Ki-value for the wild-type enzyme. The ratio of elimination to isomerization reactions is higher than in the wild-type enzyme. The KM-value for D-glyceraldehyde 3-phosphate is 10.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 2.7fold higher than the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 2280fold lower than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 3000fold lower than the value for the wild-type enzyme	Trypanosoma brucei
C14P	the KM-value for D-glyceraldehyde 3-phosphate is 94% of the value for the wild-type enzyme. The KM-value for glycerone phosphate is 58% of the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 85% of the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 81% of the value for the wild-type enzyme	Trypanosoma brucei
C14S	the KM-value for D-glyceraldehyde 3-phosphate is 1.4fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is identical to the value of the wild-type value. The turnover number for D-glyceraldehyde 3-phosphate is 1.15fold higher than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is nearly identical to the value of the wild-type enzyme	Trypanosoma brucei
C14T	the KM-value for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 79% of the value of the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 92% of the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 92% of the value of the wild-type enzyme	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Phosphoglycolate	-	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.32	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14T	Trypanosoma brucei
0.33	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14P	Trypanosoma brucei
0.35	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, wild-type enzyme	Trypanosoma brucei
0.42	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14V	Trypanosoma brucei
0.43	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14A	Trypanosoma brucei
0.5	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14S	Trypanosoma brucei
3.6	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14F	Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	mutant enzyme C14T, gel filtration	Trypanosoma brucei
26879	-	1 * 26879, equilibrium sedimentation	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type enzyme and mutant enzymes C14A, C14P, C14S, C14T, C14V and C14G. Attempts to purify mutant enzymes C14M and C14L are unsuccessful, the enzymes exhibit a strong tendency to undergo aggregation	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	-	Trypanosoma brucei	Glycerone phosphate	-	r
glycerone phosphate	-	Trypanosoma brucei	methylglyoxal + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 26879, equilibrium sedimentation	Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.9	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14F	Trypanosoma brucei
3667	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14P	Trypanosoma brucei
3670	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14P	Trypanosoma brucei
3830	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14T	Trypanosoma brucei
3833	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14T	Trypanosoma brucei
4000	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14V	Trypanosoma brucei
4330	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, wild-type enzyme	Trypanosoma brucei
4333	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, wild-type enzyme	Trypanosoma brucei
5000	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14S	Trypanosoma brucei
5167	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14A	Trypanosoma brucei
5170	-	D-glyceraldehyde 3-phosphate	pH 7.4, 25°C, mutant enzyme C14A	Trypanosoma brucei

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.024	-	2-Phosphoglycolate	wild-type enzyme	Trypanosoma brucei
0.076	-	2-Phosphoglycolate	mutant enzyme C14F	Trypanosoma brucei

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Release 2023.1 (January 2023)