Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Moritella marina |
Crystallization (Comment) | Organism |
---|---|
crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex, at 2.7 A resolution | Moritella marina |
hanging drop vapor diffusion method. Crystal structure of the enzyme-sulfate complex and the enzyme-2-phosphoglycolate complex at a 2.7 A resolution | Moritella marina |
Protein Variants | Comment | Organism |
---|---|---|
A238S | mutant enzyme A238S has a higher thermal stability than the wild-type enzyme. The turnover number of the mutant enzyme is somewhat lower than that observed for the wild-type enzyme, the Km-value for D-glyceraldehyde 2-phosphate is somewhat higher | Moritella marina |
A238S | catalytic efficiency of the mutant enzyme is somewhat reduced, and its stability is considerably increased. The half-life at 25°C is 27 min compared to 10 min for the wild-type enzyme | Moritella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phosphoglycolate | - |
Escherichia coli | |
2-Phosphoglycolate | - |
Moritella marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.03 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Escherichia coli | |
1.9 | - |
D-glyceraldehyde 3-phosphate | wild-type enzyme | Moritella marina | |
1.9 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Moritella marina | |
4.8 | - |
D-glyceraldehyde 3-phosphate | mutant A238S | Moritella marina | |
4.8 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme A238S | Moritella marina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Moritella marina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme A238S | Moritella marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Escherichia coli | Glycerone phosphate | - |
? | |
D-Glyceraldehyde 3-phosphate | - |
Moritella marina | Glycerone phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
half-life of wild-type enzyme: 58 min | Moritella marina |
25 | - |
wild-type enzyme, half-life: 10 min | Moritella marina |
25 | - |
mutant A238S, half-life: 27 min | Moritella marina |
25 | - |
half-life of wild-type enzyme: 10 min, half-life of mutant enzyme A238S: 27 min | Moritella marina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4667 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme A238S | Moritella marina | |
4670 | - |
D-glyceraldehyde 3-phosphate | mutant A238S | Moritella marina | |
4670 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme A238S | Moritella marina | |
7000 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme | Moritella marina | |
7000 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Moritella marina | |
9000 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
2-Phosphoglycolate | - |
Escherichia coli | |
89 | - |
2-Phosphoglycolate | pH 7.6, wild-type enzyme | Moritella marina | |
101 | - |
2-Phosphoglycolate | pH 7.6, mutant enzyme A238S | Moritella marina |