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Literature summary for 4.2.3.3 extracted from

  • Farsi, Z.; Pein, H.; Pazhang, M.; Zareian, S.; Ranaei-Siadat, S.O.; Khajeh, K.
    Conferral of allostery to Thermus sp. GH5 methylglyoxal synthase by a single mutation (2012), J. Biochem., 152, 531-538.
    View publication on PubMed
Search for more literature for 4.2.3.3

Protein Variants

Protein Variants Comment Organism
DELTAR97 strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.3 Thermus sp.
R97E strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.4 Thermus sp.
R97K strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 1.5 Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.
0.8
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
1.5
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C Thermus sp.
1.6
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
1.7
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C Thermus sp.
1.7
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
2.8
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C Thermus sp.
3.2
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp. B3VH91
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
 Thermus sp. methylglyoxal + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 10 min, wild-type retains 84 of initial activity, mutant R97E shows complete loss of activity Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00025
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.00025
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.0004
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C Thermus sp.
0.001
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C Thermus sp.
0.5
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.7
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C Thermus sp.
233
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
326
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00015
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.00016
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.0003
-
 dihydroxyacetone phosphate mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C Thermus sp.
0.0006
-
 dihydroxyacetone phosphate mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C Thermus sp.
0.2
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
0.3
-
 dihydroxyacetone phosphate mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C Thermus sp.
288
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C Thermus sp.
653
-
 dihydroxyacetone phosphate wild-type, Hill coefficient 1, pH 6.5, 60°C Thermus sp.