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Literature summary for 4.2.3.3 extracted from

  • Mohammadi, M.; Kashi, M.A.; Zareian, S.; Mirshahi, M.; Khajeh, K.
    Remarkable improvement of methylglyoxal synthase thermostability by His-His interaction (2014), Appl. Biochem. Biotechnol., 172, 157-167.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information a mutant carrying one additional His residue between residues Arg22 and His23 shos remarkable increase in thermostability, whereas a mutant carrying two additional His residues between residues Arg22 and His23 is very unstable. Replacing His23 in the latter mutant by Ala leads to a decrease in half-lifecompared to wild-type, but increase compared to the original mutant Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
dihydroxyacetone phosphate carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C Thermus sp.
0.83
-
dihydroxyacetone phosphate wild-type, Hill coefficient 2.2, pH 6.2, 60°C Thermus sp.
1.45
-
dihydroxyacetone phosphate carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp. B3VH91
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
Thermus sp. methylglyoxal + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
138
-
dihydroxyacetone phosphate carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C Thermus sp.
153
-
dihydroxyacetone phosphate carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C Thermus sp.
230
-
dihydroxyacetone phosphate wild-type, Hill coefficient 2.2, pH 6.2, 60°C Thermus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
95
-
dihydroxyacetone phosphate carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C Thermus sp.
227
-
dihydroxyacetone phosphate carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C Thermus sp.
275
-
dihydroxyacetone phosphate wild-type, Hill coefficient 2.2, pH 6.2, 60°C Thermus sp.