Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Thermus sp. GH5 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
glycerone phosphate | allosteric enzyme | Thermus sp. GH5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14300 | - |
6 * 14300, gel filtration, SDS-PAGE | Thermus sp. GH5 |
89000 | - |
gel filtration | Thermus sp. GH5 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. GH5 | B3VH91 | - |
- |
Purification (Comment) | Organism |
---|---|
heat shock (70°C, 15 min), anion-exchange chromatography (Q-Sepharose) | Thermus sp. GH5 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6000 | - |
- |
Thermus sp. GH5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate | - |
Thermus sp. GH5 | pyruvaldehyde + phosphate | tautomerization to methylglyoxal | ? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 14300, gel filtration, SDS-PAGE | Thermus sp. GH5 |
Synonyms | Comment | Organism |
---|---|---|
methylglyoxal synthase | - |
Thermus sp. GH5 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermus sp. GH5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
75 | 100% activity after 15 min | Thermus sp. GH5 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
325 | - |
glycerone phosphate | - |
Thermus sp. GH5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermus sp. GH5 |