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Literature summary for 4.2.3.3 extracted from

  • Marks, G.T.; Susler, M.; Harrison, D.H.
    Mutagenic studies on histidine 98 of methylglyoxal synthase: effects on mechanism and conformational change (2004), Biochemistry, 43, 3802-3813.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phosphoglycolate activates H98Q variant Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Escherichia coli

Protein Variants

Protein Variants Comment Organism
H98N 50fold decrease in kcat Escherichia coli
H98Q 250fold lower catalytic activity than wild-type enzyme, change in conformation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phosphate acts as competitive inhibitor on the H98Q variant and as an allosteric-type inhibitor on the wild-type enzyme Escherichia coli
Phosphoglycolate inhibits wild-type enzyme and activates H98Q variant Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
dihydroxyacetone phosphate pH 7, 25°C, wild-type Escherichia coli
0.26
-
dihydroxyacetone phosphate pH 7, 25°C, H98N mutant Escherichia coli
0.6
-
dihydroxyacetone phosphate pH 7, 25°C, H98Q mutant, presence of phosphoglycolate Escherichia coli
0.62
-
dihydroxyacetone phosphate pH 6, 25°C, wild-type Escherichia coli
1.3
-
dihydroxyacetone phosphate pH 7, 25°C, H98Q mutant, absence of phosphoglycolate Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A731
-
-

Purification (Commentary)

Purification (Comment) Organism
95% pure Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
glycerone phosphate = 2-oxopropanal + phosphate mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate converts dihydrocyacetone phosphate to enol pyruvaldehyde, this enol then tautomerizes to methylglyoxal in solution Escherichia coli methylglyoxal + phosphate
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.96
-
dihydroxyacetone phosphate pH 7, 25°C, H98Q mutant, absence of phosphoglycolate Escherichia coli
4.4
-
dihydroxyacetone phosphate pH 7, 25°C, H98N mutant Escherichia coli
6.08
-
dihydroxyacetone phosphate pH 7, 25°C, H98Q mutant, absence of phosphoglycolate Escherichia coli
17
-
dihydroxyacetone phosphate pH 7, 25°C, H98Q mutant, presence of phosphoglycolate Escherichia coli
179
-
dihydroxyacetone phosphate pH 6, 25°C, wild-type Escherichia coli
220
-
dihydroxyacetone phosphate pH 7, 25°C, wild-type Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
H98Q variant Escherichia coli
6.8
-
H98N variant Escherichia coli
7.5
-
wild-type enzyme Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000058
-
Phosphoglycolate pH 7, 25°C, H98N variant Escherichia coli
0.000093
-
phosphate pH 7, 25°C, H98Q variant Escherichia coli