Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phosphoglycolate | activates H98Q variant | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H98N | 50fold decrease in kcat | Escherichia coli |
H98Q | 250fold lower catalytic activity than wild-type enzyme, change in conformation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | acts as competitive inhibitor on the H98Q variant and as an allosteric-type inhibitor on the wild-type enzyme | Escherichia coli | |
Phosphoglycolate | inhibits wild-type enzyme and activates H98Q variant | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
dihydroxyacetone phosphate | pH 7, 25°C, wild-type | Escherichia coli | |
0.26 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98N mutant | Escherichia coli | |
0.6 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98Q mutant, presence of phosphoglycolate | Escherichia coli | |
0.62 | - |
dihydroxyacetone phosphate | pH 6, 25°C, wild-type | Escherichia coli | |
1.3 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98Q mutant, absence of phosphoglycolate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A731 | - |
- |
Purification (Comment) | Organism |
---|---|
95% pure | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
glycerone phosphate = 2-oxopropanal + phosphate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | converts dihydrocyacetone phosphate to enol pyruvaldehyde, this enol then tautomerizes to methylglyoxal in solution | Escherichia coli | methylglyoxal + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98Q mutant, absence of phosphoglycolate | Escherichia coli | |
4.4 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98N mutant | Escherichia coli | |
6.08 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98Q mutant, absence of phosphoglycolate | Escherichia coli | |
17 | - |
dihydroxyacetone phosphate | pH 7, 25°C, H98Q mutant, presence of phosphoglycolate | Escherichia coli | |
179 | - |
dihydroxyacetone phosphate | pH 6, 25°C, wild-type | Escherichia coli | |
220 | - |
dihydroxyacetone phosphate | pH 7, 25°C, wild-type | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
H98Q variant | Escherichia coli |
6.8 | - |
H98N variant | Escherichia coli |
7.5 | - |
wild-type enzyme | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000058 | - |
Phosphoglycolate | pH 7, 25°C, H98N variant | Escherichia coli | |
0.000093 | - |
phosphate | pH 7, 25°C, H98Q variant | Escherichia coli |