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Literature summary for 4.2.3.3 extracted from

  • Yuan, P.M.; Gracy, R.W.
    The conversion of dihydroxyacetone phosphate to methylglyoxal and inorganic phosphate by methylglyoxal synthase (1977), Arch. Biochem. Biophys., 183, 1-6.
    View publication on PubMed

General Stability

General Stability Organism
phosphate stabilizes towards both cold-induced inactivation and against heat-induced inactivation Proteus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
methylglyoxal linear noncompetitive inhibitor Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
-
-

Reaction

Reaction Comment Organism Reaction ID
glycerone phosphate = 2-oxopropanal + phosphate stereospecific deprotonation of the pro-S hydrogen at C-3 of dihydroxyacetone, the true product of the enzymatic reaction is the enol form of methylglyoxal which is ketonized in solution Proteus vulgaris

Storage Stability

Storage Stability Organism
4°C, 10 mM imidazole buffer, pH 7.0, 1 mM EDTA, 1 mM KH2PO4, 0.05% v/v 2-mercaptoethanol, 0.1-0.5 mg/ml protein, stable Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
Proteus vulgaris methylglyoxal + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
phosphate stabilizes towards both cold-induced inactivation and against heat-induced inactivation Proteus vulgaris