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Literature summary for 4.2.1.1 extracted from

  • Faridi, S.; Satyanarayana, T.
    Characteristics of recombinant alpha-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1 (2016), Int. J. Biol. Macromol., 89, 659-668 .
    View publication on PubMed

Application

Application Comment Organism
additional information carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2 to bicarbonate and protons, thus useful in mitigating green house effect by sequestering CO2 from various point sources Halalkalibacterium halodurans

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) achieving a 31.4fold enhancement in CA production for the wild-type, subcloning in Escherichia coli strain DH5alpha. About 50% of wild-type enzyme produced is secreted when recombinant Escherichia coli harboring BhCA-pET22b is cultivated in a medium with EDTA and lysozyme because of the efficient pelB leader sequence, cultvation parameters are optimized for rBhCA secretion Halalkalibacterium halodurans

Inhibitors

Inhibitors Comment Organism Structure
2,6-PDCA
-
Halalkalibacterium halodurans
2-mercaptoethanol 15% inhibition at 5 mM Halalkalibacterium halodurans
acetazolamide strong inhibition at 5 mM Halalkalibacterium halodurans
Ag+ inhibits by 17% at 5 mM, no inhibition at 1 mM Halalkalibacterium halodurans
Al3+ inhibits by 12% at 5 mM, no inhibition at 1 mM Halalkalibacterium halodurans
Co2+ inhibits by 74% at 5 mM Halalkalibacterium halodurans
Cu2+ inhibits completely at 5 mM, 66% inhibition at 1 mM Halalkalibacterium halodurans
diethyl dicarbonate strong inhibition at 5 mM Halalkalibacterium halodurans
DTT 60% inhibition at 5 mM Halalkalibacterium halodurans
EDTA
-
Halalkalibacterium halodurans
ethyl 3-(3-dimethylaminopropyl)-carbodiimide 80% inhibition at 5 mM, via irreversible modification of carboxyl groups or thepresence of catalytically active tyrosine in the enzyme molecule Halalkalibacterium halodurans
Fe2+ inhibits completely at 5 mM, 74% inhibition at 1 mM Halalkalibacterium halodurans
Fe3+ inhibits completely at 5 mM, 75% inhibition at 1 mM Halalkalibacterium halodurans
Hg2+ inhibits completely at 5 mM, 78% inhibition at 1 mM Halalkalibacterium halodurans
iodoacetic acid
-
Halalkalibacterium halodurans
N-bromosuccinimide strong inhibition at 5 mM Halalkalibacterium halodurans
N-ethylmaleimide strong inhibition at 5 mM Halalkalibacterium halodurans
Na+ inhibits by 25% at 2 mM Halalkalibacterium halodurans
Ni2+ inhibits by 84% at 5 mM, 80% inhibition at 1 mM Halalkalibacterium halodurans
Pb2+ inhibits by 30% at 5 mM Halalkalibacterium halodurans
PMSF 80% inhibition at 5 mM Halalkalibacterium halodurans
SDS
-
Halalkalibacterium halodurans
Triton X-100
-
Halalkalibacterium halodurans
Tween 80
-
Halalkalibacterium halodurans
Woodward's reagent K WRK Halalkalibacterium halodurans
Zn2+ inhibits by 95% at 5 mM Halalkalibacterium halodurans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans
additional information no effect on enzyme activity at 5 mM by Ca2+, NH4+, Mg2+, Ba2+, and Mn2+ Halalkalibacterium halodurans
Sn2+ activates about 40% at 5 mM Halalkalibacterium halodurans
Zn2+ required, metalloenzyme Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
about Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Halalkalibacterium halodurans
-
H2CO3
-
r
CO2 + H2O Halalkalibacterium halodurans TSLV1
-
H2CO3
-
r
H2CO3 Halalkalibacterium halodurans
-
CO2 + H2O
-
r
H2CO3 Halalkalibacterium halodurans TSLV1
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans A0A0N9WRG3
-
-
Halalkalibacterium halodurans TSLV1 A0A0N9WRG3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) medium by nickel affinity chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Halalkalibacterium halodurans H2CO3
-
r
CO2 + H2O
-
Halalkalibacterium halodurans TSLV1 H2CO3
-
r
H2CO3
-
Halalkalibacterium halodurans CO2 + H2O
-
r
H2CO3
-
Halalkalibacterium halodurans TSLV1 CO2 + H2O
-
r
additional information mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans ?
-
?
additional information mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans TSLV1 ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Halalkalibacterium halodurans
More structural secondary composition of rBhCA at different pH values, overview Halalkalibacterium halodurans

Synonyms

Synonyms Comment Organism
alpha carbonic anhydrase
-
Halalkalibacterium halodurans
alpha-carbonic anhydrase
-
Halalkalibacterium halodurans
BhCA
-
Halalkalibacterium halodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Halalkalibacterium halodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 72 Tm of BhCA is 72°C, T1/2 values at 50°C and 60°C are 66 min and 24 min, respectively Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
assay at Halalkalibacterium halodurans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 purified recombinant enzyme, completely stable within this range for 48 h Halalkalibacterium halodurans

General Information

General Information Comment Organism
additional information residues H137, H139, H156, and H110 present in the active site play an important role in catalysis, the active site of recombinant BhCA is also composed of conserved Glu and Asp acid residues Halalkalibacterium halodurans
physiological function carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2to bicarbonate and protons, and an alkalistable and moderately thermostable alpha-carbonic anhydrase Halalkalibacterium halodurans