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Literature summary for 4.2.1.1 extracted from

  • West, D.; Kim, C.U.; Tu, C.; Robbins, A.H.; Gruner, S.M.; Silverman, D.N.; McKenna, R.
    Structural and kinetic effects on changes in the CO(2) binding pocket of human carbonic anhydrase II (2012), Biochemistry, 51, 9156-9163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystallography is determined at 1.7-1.5 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
V143A mutant shows 20fold decreased catalytic activity compared to wild-type Homo sapiens
V143I mutant shows 20fold decreased catalytic activity compared to wild-type Homo sapiens
V143L mutant shows 20fold decreased catalytic activity compared to wild-type Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Homo sapiens
-
H2CO3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Homo sapiens H2CO3
-
?

Synonyms

Synonyms Comment Organism
hCAII
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000005
-
CO2 mutant V143A, pH 8.3, 25°C Homo sapiens
0.0000007
-
CO2 mutant V143I, pH 8.3, 25°C Homo sapiens
0.000001
-
CO2 wild-type, pH 8.3, 25°C Homo sapiens
0.0000011
-
CO2 mutant V143L, pH 8.3, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5500
-
CO2 mutant V143L, pH 8.3, 25°C Homo sapiens
9300
-
CO2 mutant V143I, pH 8.3, 25°C Homo sapiens
42000
-
CO2 mutant V143A, pH 8.3, 25°C Homo sapiens
120000
-
CO2 wild-type, pH 8.3, 25°C Homo sapiens