Cloned (Comment) | Organism |
---|---|
mutants are expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutants are crystallized by hanging drop vapour diffusion method, with 50 mM Tris-HCl (pH 9.0) and 1.3 M sodium citrate at 20°C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N62A | the mutant shows a predominantly inward orientation of the side chain of His64 | Homo sapiens |
N62D | the mutant shows a predominantly outward orientation of the side chain of His64 | Homo sapiens |
N62L | the mutant shows a predominantly inward orientation of the side chain of His64 | Homo sapiens |
N62T | the mutant shows both inward and outward orientation of the side chain of His64 like the wild type enzyme | Homo sapiens |
N62V | the mutant shows a predominantly inward orientation of the side chain of His64 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloenzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | Homo sapiens | - |
H2CO3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00918 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
? | |
CO2 + H2O | - |
Homo sapiens | H2CO3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CA II | - |
Homo sapiens |
carbonic anhydrase II | - |
Homo sapiens |