Literature summary for 4.1.2.19 extracted from

Oroz-Guinea, I.; Sanchez-Moreno, I.; Mena, M.; Garcia-Junceda, E.
Hyperthermophilic aldolases as biocatalyst for C-C bond formation rhamnulose 1-phosphate aldolase from Thermotoga maritima (2015), Appl. Microbiol. Biotechnol., 99, 3057-3068 .

Search for more literature for 4.1.2.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.6	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	maximum activity at 0.1875 mM	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
114000	-	gel filtration	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Glycerone phosphate + (S)-lactaldehyde	Thermotoga maritima	-	L-Rhamnulose 1-phosphate	-	r
L-Rhamnulose 1-phosphate	Thermotoga maritima	-	Glycerone phosphate + (S)-lactaldehyde	-	r

Organic Solvent Stability

Organic Solvent	Comment	Organism
acetonitrile	the enzyme retains 90 % of its activity in the presence of 40 % (v/v) of acetonitrile	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-IDA agarose column chromatography	Thermotoga maritima

Storage Stability

Storage Stability	Organism
25°C, 28 days, about 30% loss of activity	Thermotoga maritima
4°C, 28 days, less than 20% loss of activity	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Glycerone phosphate + (S)-lactaldehyde	-	Thermotoga maritima	L-Rhamnulose 1-phosphate	-	r
Glycerone phosphate + (S)-lactaldehyde	more than 90% aldol formation at 45°C	Thermotoga maritima	L-Rhamnulose 1-phosphate	-	r
glycerone phosphate + benzyloxyacetaldehyde	68% aldol formation at 45°C	Thermotoga maritima	(3R,4S)-5-(benzyloxy)-3,4-dihydroxy-2-oxopentyl phosphate	-	?
glycerone phosphate + ethyl-3-methyl-4-oxocrotonate	87% aldol formation at 45°C	Thermotoga maritima	(3R,4S,5E)-7-ethoxy-3,4-dihydroxy-5-methyl-2,7-dioxohept-5-en-1-yl phosphate	-	?
glycerone phosphate + phenylacetaldehyde	53% aldol formation at 45°C	Thermotoga maritima	(3R,4S)-3,4-dihydroxy-2-oxo-5-phenylpentyl phosphate	-	?
L-Rhamnulose 1-phosphate	-	Thermotoga maritima	Glycerone phosphate + (S)-lactaldehyde	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 29000, SDS-PAGE	Thermotoga maritima
homotetramer	4 * 28900, calculated from amino acid sequence	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
Rha-1PA	-	Thermotoga maritima
TM1072	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
95	-	-	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	115	the enzyme shows a half-life of 44 h, 33 h and 3.5 h at 80°C, 95°C and 115°C, respectively	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.52	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.7	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

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Release 2023.1 (January 2023)