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Literature summary for 4.1.2.17 extracted from
- Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic archaeon Methanococcus jannaschii (2012), Enzyme Microb. Technol., 50, 209-214.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-psicose 1-phosphate | product inhibition is noncompetitive vs. dihydroxyacetone phosphate and competitive vs. DL-glyceraldehyde. Product inhibition is uncompetitive against DHAP at saturated DL-glyceraldehyde | Methanocaldococcus jannaschii |  |
| dihydroxyacetone phosphate | - |
Methanocaldococcus jannaschii | |
| DL-glyceraldehyde | - |
Methanocaldococcus jannaschii | |
| DL-threose | competitive with respect to dihydroxyacetone phosphate and uncompetitive with respect to DL-glyceraldehyde; the dead-end inhibition patterns as assessed by varying the concentration of dihydroxyacetone phosphate at several fixed concentrations of DL-threose is uncompetitive against dihydroxyacetone phosphate at the DL-glyceraldehyde Km concentration, and the dead-end inhibition by varying the concentrations of DL-threose is competitive against DL-glyceraldehyde at the DHAP Km concentration | Methanocaldococcus jannaschii | |
| L-Tagatose 1-phosphate | product inhibition is noncompetitive vs. dihydroxyacetone phosphate and competitive vs. DL-glyceraldehyde. Product inhibition is uncompetitive against DHAP at saturated DL-glyceraldehyde | Methanocaldococcus jannaschii | |
| trimethyl phosphonoacetate | competitive with respect to dihydroxyacetone phosphate and uncompetitive with respect to DL-glyceraldehyde; dead-end inhibition as assessed by varying the dihydroxyacetone phosphate concentration at several fixed concentrations of trimethyl phosphonoacetat: in the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. dihydroxyacetone phosphate at the DL-glyceraldehyde Km concentration (0.74 mM). In the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. DL-glyceraldehyde at the dihydroxyacetone phosphate Km concentration (0.091 mM). The dead-end inhibition patterns as assessed by varying the concentration of DL-glyceraldehyde at several fixed concentrations of trimethyl phosphonoacetate: in the direction of the aldol addition reaction, trimethyl phosphonoacetate is competitive vs. DL-glyceraldehyde at the dihydroxyacetone phosphate Km concentration | Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.091 | - |
dihydroxyacetone phosphate | pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.091 | - |
dihydroxyacetone phosphate | pH not specified in the publication, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.743 | - |
DL-glyceraldehyde | pH not specified in the publication, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.743 | - |
D-glyceraldehyde | assay with DL-glyceraldehyde, pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.743 | - |
L-glyceraldehyde | assay with DL-glyceraldehyde, pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | contains a catalytically active Zn2+ ion | Methanocaldococcus jannaschii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | Q58813 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | Q58813 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0133 | - |
in the direction of hydrolysis, pH and temperature not specified in the publication | Methanocaldococcus jannaschii |
| 0.0256 | - |
in the direction of aldol condensation, pH and temperature not specified in the publication | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone phosphate + D-glyceraldehyde | kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to D-glyceraldehyde | Methanocaldococcus jannaschii | D-psicose 1-phosphate | - |
? | |
| dihydroxyacetone phosphate + D-glyceraldehyde | kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to D-glyceraldehyde | Methanocaldococcus jannaschii DSM 2661 | D-psicose 1-phosphate | - |
? | |
| dihydroxyacetone phosphate + DL-glyceraldehyde | - |
Methanocaldococcus jannaschii | L-tagatose 1-phosphate | - |
r | |
| dihydroxyacetone phosphate + DL-glyceraldehyde | - |
Methanocaldococcus jannaschii DSM 2661 | L-tagatose 1-phosphate | - |
r | |
| dihydroxyacetone phosphate + L-glyceraldehyde | kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to L-glyceraldehyde | Methanocaldococcus jannaschii | L-tagatose 1-phosphate | - |
? | |
| dihydroxyacetone phosphate + L-glyceraldehyde | kinetic mechanism is sequential, ordered addition of dihydroxyacetone phosphate prior to L-glyceraldehyde | Methanocaldococcus jannaschii DSM 2661 | L-tagatose 1-phosphate | - |
? | |
| dihydroxyacetone phosphate + L-lactaldehyde | - |
Methanocaldococcus jannaschii | L-fuculose-1-phosphate | - |
r | |
| dihydroxyacetone phosphate + L-lactaldehyde | - |
Methanocaldococcus jannaschii DSM 2661 | L-fuculose-1-phosphate | - |
r | |
| DL-glycerol 3-phosphate + DL-glyceraldehyde | - |
Methanocaldococcus jannaschii | D-psicose 1-phosphate | - |
r | |
| additional information | rapid equilibrium ordered mechanism in which dihydroxyacetone phosphate is added and bound to the enzyme prior to DL-glyceraldehyde | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | rapid equilibrium ordered mechanism in which dihydroxyacetone phosphate is added and bound to the enzyme prior to DL-glyceraldehyde | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FucA | - |
Methanocaldococcus jannaschii |
| fuculose-1-phosphate aldolase | - |
Methanocaldococcus jannaschii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
DL-glyceraldehyde | direction of aldol condensation, pH not specified in the publication, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 0.0123 | - |
dihydroxyacetone phosphate | direction of aldol condensation, pH not specified in the publication, temperature not specified in the publication | Methanocaldococcus jannaschii | |
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