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Literature summary for 4.1.2.17 extracted from

  • Ghalambor, M.A.; Heath, E.C.
    L-Fuculose 1-phosphate aldolase (1966), Methods Enzymol., 9, 538-542.
No PubMed abstract available
Search for more literature for 4.1.2.17

General Stability

General Stability Organism
10-20% of the activity is lost over a period of several months of storage with repeated freezing and thawing Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 L-Fuculose 1-phosphate
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli 0111-B4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-16°C, 10-20% of the activity is lost over a period of several months of storage with repeated freezing and thawing Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-Deoxy-L-sorbose 1-phosphate about 5% of the activity with L-fuculose 1-phosphate Escherichia coli ?
-
 ?
D-Fructose 1,6-diphosphate about 4% of the activity with L-fuculose 1-phosphate Escherichia coli ?
-
 ?
D-Fructose 1-phosphate about 4% of the activity with L-fuculose 1-phosphate Escherichia coli ?
-
 ?
D-Ribulose 1,5-diphosphate about 6% of the activity with L-fuculose 1-phosphate Escherichia coli ?
-
 ?
Glycerone phosphate + (R)-lactaldehyde at 73% of the activity with (S)-lactaldehyde Escherichia coli 6-Deoxy-D-psicose 1-phosphate
-
 ?
Glycerone phosphate + (R)-lactaldehyde at 73% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 6-Deoxy-D-psicose 1-phosphate
-
 ?
Glycerone phosphate + (S)-lactaldehyde
-
 Escherichia coli L-Fuculose 1-phosphate
-
 ?
Glycerone phosphate + (S)-lactaldehyde
-
 Escherichia coli 0111-B4 L-Fuculose 1-phosphate
-
 ?
glycerone phosphate + D-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli D-psicose 1-phosphate
-
 ?
glycerone phosphate + D-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 D-psicose 1-phosphate
-
 ?
Glycerone phosphate + glycolaldehyde at 10% of the activity with (S)-lactaldehyde Escherichia coli D-Ribulose 1-phosphate
-
 ?
Glycerone phosphate + glycolaldehyde at 10% of the activity with (S)-lactaldehyde Escherichia coli 0111-B4 D-Ribulose 1-phosphate
-
 ?
glycerone phosphate + L-glyceraldehyde at 33% of the activity with (S)-lactaldehyde Escherichia coli L-tagatose 1-phosphate
-
 ?
L-fuculose 1-phosphate
-
 Escherichia coli glycerone phosphate + (S)-lactaldehyde
-
 ?
L-fuculose 1-phosphate
-
 Escherichia coli 0111-B4 glycerone phosphate + (S)-lactaldehyde
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.2
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 approximately 50% of maximal activity at pH 6.5 and at pH 8 Escherichia coli
6.5 8.2 approximately 50% of maximal activity at pH 6.5 and at pH 8.2 Escherichia coli