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Literature summary for 4.1.2.13 extracted from

  • Droppelmann, C.A.; Saez, D.E.; Asenjo, J.L.; Yanez, A.J.; Garcia-Rocha, M.; Concha, I.I.; Grez, M.; Guinovart, J.J.; Slebe, J.C.
    A new level of regulation in gluconeogenesis metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase (2015), Biochem. J., 472, 225-237 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Aldob, recombinant expression of GST-tagged liver enzyme in Escherichia coli strain BL21(DE3) Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm mainly Rattus norvegicus 5737
-
additional information liver fructose 1,6-bisphosphatase (FBPase-1) guides the cellular localization of aldolase B Rattus norvegicus
-
-
nucleus
-
Rattus norvegicus 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Rattus norvegicus
38000
-
gel filtration, recombinant enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Rattus norvegicus
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-fructose 1,6-bisphosphate + H2O Rattus norvegicus Wistar
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P00884
-
-
Rattus norvegicus Wistar P00884
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
recombinant GST-tagged liver enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver aldolase B is the predominant isozyme in liver and is also expressed in kidney Rattus norvegicus
-
additional information coexpression of fructose 1,6-bisphosphate aldolase B and fructose 1,6-bisphosphatase (FBPase-1) in cultured cells Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Rattus norvegicus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-fructose 1,6-bisphosphate + H2O
-
Rattus norvegicus Wistar glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 38000, recombinant enzyme, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
Aldob
-
Rattus norvegicus
aldolase B
-
Rattus norvegicus
F1,6-P2 aldolase
-
Rattus norvegicus
fructose-1,6-bisphosphate aldolase B
-
Rattus norvegicus

General Information

General Information Comment Organism
drug target the enzyme (FBPase-1) is considered to be a new target for the control of diabetes Rattus norvegicus
metabolism metabolic conditions modulate aldolase B and FBPase-1 activity at the cellular level through the regulation of their interaction, suggesting that their association confers a catalytic advantage for both enzymes Rattus norvegicus
physiological function fructose-1,6-bisphosphate aldolase B catalyses the reversible reaction of glyceraldehyde-3-phosphate (G3-P) and dihydroxyacetone phosphate (DHAP) to form fructose 1,6-bisphosphate and participates in both gluconeogenesis and glycolysis. In vitro protein-protein interaction analysis between liver fructose 1,6-bisphosphate aldolase B and liver fructose 1,6-bisphosphatase (FBPase-1) shows a specific and regulable interaction between them, whereas aldolase A (muscle isozyme) and FBPase-1 show no interaction, by real-time interaction analysis by surface plasmon resonance. The interaction between aldolase B and FBPase-1 is specific and reversible. The affinity of the aldolase B and FBPase-1 complex is modulated by intermediate metabolites, but only in the presence of K+. A decreased association constant is observed in the presence of adenosine monophosphate, fructose-2,6-bisphosphate, fructose-6-phosphate and inhibitory concentrations of fructose-1,6-bisphosphate. Conversely, the association constant of the complex increases in the presence of dihydroxyacetone phosphate (DHAP) and non-inhibitory concentrations of fructose-1,6-bisphosphate. FBPase-1 guides the cellular localization of aldolase B Rattus norvegicus