Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Star (DE3) cells | Toxoplasma gondii |
expression in Escherichia coli | Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
crystals are grown at 20°C by hanging-drop vapor diffusion using a 1:1 mixture of protein/precipitant. The enzyme crystallizes with one tetrameric subunit in the asymmetric unit of space group P4(2)2(1)2, adopting the classic (alpha/beta)8-barrel fold | Toxoplasma gondii |
hanging drop vapor diffusion method, using 0.1 M sodium acetate, pH 5.5, 0.2 M lithium sulfate, 3.5% (w/v) PEG 8000, and 10% (w/v) PEG 1000 | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tagatose 1,6-bisphosphate | competitive inhibitor | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00405 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 25°C | Toxoplasma gondii | |
0.0045 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Toxoplasma gondii | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | Toxoplasma gondii | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q8I8I2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Toxoplasma gondii |
HisTrap column chromatography and Superdex gel filtration | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
Toxoplasma gondii | D-Fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Toxoplasma gondii |
class I fructose-1,6-bisphosphate aldolase | - |
Toxoplasma gondii |
FBP aldolase | - |
Toxoplasma gondii |
fructose-1,6-bisphosphate aldolase | - |
Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.09 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 25°C | Toxoplasma gondii | |
7.09 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C | Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
physiological function | glycolytic enzyme | Toxoplasma gondii |