Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | causes further enhancement of enzyme activity when added iin 1-10 mM amounts in the standard assay medium containing KCl. Activity with 12 mM arsenate, is threefold higher than the original aldolase activity | Haloarcula vallismortis | |
cysteine | 1 mM, 1.3fold activation | Haloarcula vallismortis | |
diphosphate | causes further enhancement of enzyme activity when added in 1-10 mM amounts in the standard assay medium containing KCl | Haloarcula vallismortis | |
phosphate | causes further enhancement of enzyme activity when added in 1-10 mM amounts in the standard assay medium containing KCl | Haloarcula vallismortis | |
reduced glutathione | 1 mM, 1.4fold activation | Haloarcula vallismortis |
General Stability | Organism |
---|---|
the enzyme is totally inactivated within 75 min in 0.5 - 0.05 mM Tris/HCI pH 8.0, whereas 80% activity is retained in 3 M KCl, 50 mM Tris/HCI pH 8.0 at the end of 72 h. Addition of 1 M (NH4)2S04 to the enzyme in 1.5 M KCI stabilizes the enzyme activity for at least 48 h | Haloarcula vallismortis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | - |
Haloarcula vallismortis | |
5,5'-dithiobis(2-nitrobenzoic acid) | 1 mM, complete inhibition | Haloarcula vallismortis | |
CuCl2 | 1 mM, complete inhibition | Haloarcula vallismortis | |
EDTA | 1 mM, 7% inhibition | Haloarcula vallismortis | |
HgCl2 | 1 mM, complete inhibition | Haloarcula vallismortis | |
iodoacetate | 1 mM, 46% inhibition | Haloarcula vallismortis | |
NaNO3 | - |
Haloarcula vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 37°C | Haloarcula vallismortis | |
28 | - |
D-Fructose 1-phosphate | pH 7.5, 37°C | Haloarcula vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the enzyme activity is enhanced by increasing KCl and NaCl concentrations. Optimum activity is diaplayed in 2.5 M KCl, while that in equimolar NaCl is lower. However, aldolase is less active in NH4CI and LiCI | Haloarcula vallismortis | |
LiCl | the enzyme activity is enhanced by increasing KCl and NaCl concentrations. Optimum activity is diaplayed in 2.5 M KCl, while that in equimolar NaCl is lower. However, aldolase is less active in NH4CI and LiCI | Haloarcula vallismortis | |
NaCl | the enzyme activity is enhanced by increasing KCl and NaCl concentrations. Optimum activity is diaplayed in 2.5 M KCl, while that in equimolar NaCl is lower. However, aldolase is less active in NH4CI and LiCI | Haloarcula vallismortis | |
NH4Cl | the enzyme activity is enhanced by increasing KCl and NaCl concentrations. Optimum activity is diaplayed in 2.5 M KCl, while that in equimolar NaCl is lower. However, aldolase is less active in NH4CI and LiCI | Haloarcula vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
10 * 27000 | Haloarcula vallismortis |
280000 | - |
gel filtration | Haloarcula vallismortis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula vallismortis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula vallismortis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.4 | - |
pH 7.5, 37°C | Haloarcula vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Haloarcula vallismortis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-Fructose 1-phosphate | the D-fructose 1,6-bisphosphate/D-fructose 1-phosphate cleavage ratio is 55:1 | Haloarcula vallismortis | Glycerone phosphate + D-glyceraldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 27000 | Haloarcula vallismortis |
Synonyms | Comment | Organism |
---|---|---|
fructose-1,6-bisphosphate aldolase | - |
Haloarcula vallismortis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloarcula vallismortis |
41 | - |
- |
Haloarcula vallismortis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
aldolase activity, which is lost within 2 min at 56°C, can be retained for 30 min at the same temperature in the presence of 100 mM phosphate | Haloarcula vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haloarcula vallismortis |