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Literature summary for 4.1.2.13 extracted from

  • Nakahara, K.; Yamamoto, H.; Miyake, C.; Yokota, A.
    Purification and characterization of class-I and class-II fructose-1,6-bisphosphate aldolases from the cyanobacterium Synechocystis sp. PCC 6803 (2003), Plant Cell Physiol., 44, 326-333.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-ribulose 1,5-bisphosphate
-
Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
D-fructose 1,6-bisphosphate pH 8, class I aldolase, recombinant enzyme Synechocystis sp.
0.008
-
D-fructose 1,6-bisphosphate pH 7.5, class II aldolase, purified enzyme Synechocystis sp.
0.008
-
D-fructose 1,6-bisphosphate pH 7.5, class II aldolase, recombinant enzyme Synechocystis sp.
0.008
-
sedoheptulose 1,7-bisphosphate pH 7.5, class II aldolase, recombinant enzyme Synechocystis sp.
0.009
-
D-fructose 1,6-bisphosphate pH 8, class I aldolase, purified enzyme Synechocystis sp.
0.047
-
sedoheptulose 1,7-bisphosphate pH 8, class I aldolase, recombinant enzyme Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ best divalent cation, optimal concentration: 0.2 mM Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Synechocystis sp.
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
class I and class II aldolase Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.77
-
class II aldolase Synechocystis sp.
8.62
-
class I aldolase Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Synechocystis sp. glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r
sedoheptulose 1,7-bisphosphate
-
Synechocystis sp. ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 class I aldolase Synechocystis sp.
7 7.5 class II aldolase Synechocystis sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
D-ribulose 1,5-bisphosphate pH 7.5, class II aldolase, purified enzyme Synechocystis sp.
0.37
-
D-ribulose 1,5-bisphosphate pH 8, class I aldolase, recombinant enzyme Synechocystis sp.
0.59
-
D-ribulose 1,5-bisphosphate pH 7.5, class II aldolase, recombinant enzyme Synechocystis sp.