Literature summary for 4.1.2.13 extracted from

Pezza, J.A.; Choi, K.H.; Berardini, T.Z.; Beernink, P.T.; Allen, K.N.; Tolan, D.R.
Spatial clustering of isozyme-specific residues reveals unlikely determinants of isozyme specificity in fructose-1,6-bisphosphate aldolase (2003), J. Biol. Chem., 278, 17307-17313.

Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
-	Oryctolagus cuniculus

Protein Variants

Protein Variants	Comment	Organism
H156E	similar kinetics and stability as wild-type enzyme	Oryctolagus cuniculus
additional information	AB_All mutant, isozyme-specific residues from aldolase B swapped into aldolase A and vice versa, decreased Km and kcat compared to aldolase A, similar Km and kcat to aldolase B	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00084	-	D-fructose 1,6-bisphosphate	30°C, aldolase B	Oryctolagus cuniculus
0.0011	-	D-fructose 1,6-bisphosphate	30°C, AB_All mutant	Oryctolagus cuniculus
0.0095	-	D-fructose 1,6-bisphosphate	30°C, aldolase A	Oryctolagus cuniculus
0.0102	-	D-fructose 1,6-bisphosphate	30°C, H156E mutant	Oryctolagus cuniculus
0.723	-	D-Fructose 1-phosphate	30°C, aldolase B	Oryctolagus cuniculus
2.6	-	D-Fructose 1-phosphate	30°C, AB_All mutant	Oryctolagus cuniculus
39.8	-	D-Fructose 1-phosphate	30°C, aldolase A	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 1,6-bisphosphate	Oryctolagus cuniculus	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	P00883	rabbit	-

Purification (Commentary)

Purification (Comment)	Organism
-	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Oryctolagus cuniculus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-Fructose 1-phosphate	-	Oryctolagus cuniculus	Glycerone phosphate + D-glyceraldehyde	-	?

Synonyms

Synonyms	Comment	Organism
ClassII FBP-aldolase	-	Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.63	-	D-Fructose 1-phosphate	30°C, aldolase A	Oryctolagus cuniculus
1.07	-	D-fructose 1,6-bisphosphate	30°C, AB_All mutant	Oryctolagus cuniculus
1.47	-	D-Fructose 1-phosphate	30°C, AB_All mutant	Oryctolagus cuniculus
1.53	-	D-fructose 1,6-bisphosphate	30°C, aldolase B	Oryctolagus cuniculus
2.94	-	D-fructose 1,6-bisphosphate	30°C, aldolase B	Oryctolagus cuniculus
6.08	-	D-fructose 1,6-bisphosphate	30°C, AB_All mutant	Oryctolagus cuniculus
6.08	-	D-Fructose 1-phosphate	30°C, AB_All mutant	Oryctolagus cuniculus
6.08	-	D-Fructose 1-phosphate	30°C, aldolase A	Oryctolagus cuniculus
6.08	-	D-Fructose 1-phosphate	30°C, aldolase B	Oryctolagus cuniculus
10.2	-	D-fructose 1,6-bisphosphate	30°C, aldolase A	Oryctolagus cuniculus
16.5	-	D-fructose 1,6-bisphosphate	30°C, H156E mutant	Oryctolagus cuniculus

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Release 2023.1 (January 2023)