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Literature summary for 4.1.2.13 extracted from

  • Choi, K.H.; Shi, J.; Hopkins, C.E.; Tolan, D.R.; Allen, K.N.
    Snapshots of catalysis: the structure of fructose-1,6-(bis)phosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate (2001), Biochemistry, 40, 13868-13875.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
complex of aldolase and dihydroxyacetone phosphate Schiff-base, hanging drop vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
C239A activity similar to wild-type enzyme, more stable in long-term storage at 25°C Homo sapiens
C289A activity similar to wild-type enzyme, more stable in long-term storage at 25°C Homo sapiens
C338A activity similar to wild-type enzyme, more stable in long-term storage at 25°C Homo sapiens
C72A activity similar to wild-type enzyme, more stable in long-term storage at 25°C Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
D-fructose 1,6-bisphosphate wild-type and mutants, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Homo sapiens
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
95% pure Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate mechanism Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
D-fructose 1,6-bisphosphate wild-type and mutants, pH 7.4 Homo sapiens