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Literature summary for 4.1.2.13 extracted from

  • Zhang, R.; Kusakabe, T.; Iwanaga, N.; Sugimoto, Y.; Kondo, K.; Takasaki, Y.; Imai, T.; Yoshida, M.; Hori, K.
    Lamprey fructose-1,6-bisphosphate aldolase: characterization of the muscle-type and non-muscle-type isozymes (1997), Arch. Biochem. Biophys., 341, 170-176.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Lethenteron camtschaticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
D-fructose 1,6-bisphosphate non-muscle-type enzyme Lethenteron camtschaticum
0.005
-
D-fructose 1,6-bisphosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
0.016
-
D-fructose 1,6-bisphosphate recombinant muscle-type enzyme Lethenteron camtschaticum
0.018
-
D-fructose 1,6-bisphosphate muscle-type enzyme Lethenteron camtschaticum
8.3
-
fructose 1-phosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
9.3
-
fructose 1-phosphate muscle-type enzyme Lethenteron camtschaticum
11
-
fructose 1-phosphate non-muscle-type enzyme and recombinant muscle-type enzyme Lethenteron camtschaticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Lethenteron camtschaticum
160000
-
glycerol density gradient centrifugation Lethenteron camtschaticum

Organism

Organism UniProt Comment Textmining
Lethenteron camtschaticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lethenteron camtschaticum

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Lethenteron camtschaticum
-
muscle
-
Lethenteron camtschaticum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.92
-
muscle-type enzyme Lethenteron camtschaticum
7.01
-
recombinant non-muscle-type enzyme Lethenteron camtschaticum
17.27
-
liver enzyme Lethenteron camtschaticum
18.5
-
recombinat muscle-type enzyme Lethenteron camtschaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Lethenteron camtschaticum glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-Fructose 1-phosphate muscle-type enzyme: at 3% of the activity with fructose 1,6-diphosphate Lethenteron camtschaticum Glycerone phosphate + D-glyceraldehyde
-
?
D-Fructose 1-phosphate non-muscle-type enzyme: at about 23% of the activity with fructose 1,6-diphosphate Lethenteron camtschaticum Glycerone phosphate + D-glyceraldehyde
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Lethenteron camtschaticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.38
-
fructose 1-phosphate muscle-type enzyme Lethenteron camtschaticum
1.55
-
fructose 1-phosphate recombinant muscle-type enzyme Lethenteron camtschaticum
3.6
-
fructose 1-phosphate non-muscle-type enzyme Lethenteron camtschaticum
3.65
-
fructose 1-phosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
15.8
-
D-fructose 1,6-bisphosphate non-muscle-type enzyme Lethenteron camtschaticum
17.9
-
D-fructose 1,6-bisphosphate recombinant non-muscle-type enzyme Lethenteron camtschaticum
46.1
-
D-fructose 1,6-bisphosphate muscle-type enzyme Lethenteron camtschaticum
49.3
-
D-fructose 1,6-bisphosphate recombinant muscle-type enzyme Lethenteron camtschaticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Lethenteron camtschaticum