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Literature summary for 4.1.2.13 extracted from
- D-Fructose 1,6-bisphosphate aldolase from the dipteran Ceratitis capitata (1978), Arch. Biochem. Biophys., 188, 456-465.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0043 | - |
D-fructose 1,6-bisphosphate | - |
Ceratitis capitata |  |
| 0.057 | - |
D-glyceraldehyde 3-phosphate | - |
Ceratitis capitata | |
| 0.065 | - |
glycerone phosphate | - |
Ceratitis capitata | |
| 1.5 | - |
fructose 1-phosphate | - |
Ceratitis capitata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Ceratitis capitata |
| 159000 | - |
gel filtration | Ceratitis capitata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ceratitis capitata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ceratitis capitata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.9 | - |
- |
Ceratitis capitata |
| 11 | - |
- |
Ceratitis capitata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Ceratitis capitata | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-Fructose 1-phosphate | - |
Ceratitis capitata | Glycerone phosphate + D-glyceraldehyde | - |
? | |
| Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
Ceratitis capitata | D-Fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Ceratitis capitata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
phosphate and 2-mercaptoethanol protect against thermal inactivation | Ceratitis capitata |
| 37 | - |
pH 7.4, 30 min, stable | Ceratitis capitata |
| 65 | - |
10 min, complete inactivation | Ceratitis capitata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
cleavage of fructose 1-phosphate | Ceratitis capitata |
| 7.4 | - |
cleavage of D-fructose 1,6-bisphosphate | Ceratitis capitata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 40% of maximal activity, pH 9.0: about 30% of maximal activity | Ceratitis capitata |
| 6 | 9.5 | about 30% of maximal activity at pH 6.0 and at pH 9.5, D-fructose 1,6-bisphosphate cleavage | Ceratitis capitata |
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Release 2023.1 (January 2023)
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