Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anti-carboxylase antiserum | effect of anti-carboxylase antiserum on carboxylase activity: under the conditions employed the carboxylation is inhibited by 80%, with parallel inhibition of CO2 incorporation into FLEEL and proPT28 (synthetic peptide) | Bos taurus | |
additional information | in the presence of high concentrations of propeptide, only minimal carboxylase activity is measurable. Antibodies to the protein inhibit the carboxylase activity in crude preparations | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
microsome | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | calcium-binding protein | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
single major band on SDS gel electrophoresis. The eluted protein contains both stable vitamin K-dependent carboxylase and vitamin K epoxidase activity | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + CO2 + O2 + vitamin K hydroquinone | Bos taurus | - |
gamma-carboxy L-glutamate + vitamin K epoxide + H2O | - |
? | |
additional information | Bos taurus | enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q07175 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities of the partially purified microsomes and the homogeneous vitamin K-dependent carboxylase are 2.77 and 279.6 nmol of vitamin K epoxide per h per mg of protein, a 101fold purification of the vitamin K epoxidase activity from partially purified microsomes | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + vitamin K hydroquinone | - |
Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
L-glutamate + CO2 + O2 + vitamin K hydroquinone | - |
Bos taurus | gamma-carboxy L-glutamate + vitamin K epoxide + H2O | - |
? | |
additional information | enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins | Bos taurus | ? | - |
? | |
proPT28 + CO2 + O2 + vitamin K hydroquinone | proPT28: synthetic peptide is designated by the following nomenclature: pro indicates the presence of the propeptide sequence, PT indicates prothrombin, the protein on which the peptide sequence is based, and 28 indicates the number of amino acid residues in the peptide | Bos taurus | ? + vitamin K epoxide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 77000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
glutamate carboxylase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | - |
Bos taurus |