Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.90 extracted from

  • Hubbard, B.R.; Ulrich, M.M.W.; Jacobs, M.; Vermeer, C.; Walsh, C.; Furie, B.; Furie, B.C.
    Vitamin K-dependent carboxylase: affinity purification from bovine liver by using a synthetic propeptide containing the gamma-carboxylation recognition site (1989), Proc. Natl. Acad. Sci. USA, 86, 6893-6897.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.90

Inhibitors

Inhibitors Comment Organism Structure
anti-carboxylase antiserum effect of anti-carboxylase antiserum on carboxylase activity: under the conditions employed the carboxylation is inhibited by 80%, with parallel inhibition of CO2 incorporation into FLEEL and proPT28 (synthetic peptide) Bos taurus
additional information in the presence of high concentrations of propeptide, only minimal carboxylase activity is measurable. Antibodies to the protein inhibit the carboxylase activity in crude preparations Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information calcium-binding protein Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 single major band on SDS gel electrophoresis. The eluted protein contains both stable vitamin K-dependent carboxylase and vitamin K epoxidase activity Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + CO2 + O2 + vitamin K hydroquinone Bos taurus
-
 gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?
additional information Bos taurus enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus Q07175
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activities of the partially purified microsomes and the homogeneous vitamin K-dependent carboxylase are 2.77 and 279.6 nmol of vitamin K epoxide per h per mg of protein, a 101fold purification of the vitamin K epoxidase activity from partially purified microsomes Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FLEEL + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus ? + vitamin K epoxide + H2O
-
 ?
L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?
additional information enzyme catalyzes the posttranslational modification of specific glutamic acid residues to form gamma-carboxygutamic acid residues within the vitamin K-dependent proteins Bos taurus ?
-
 ?
proPT28 + CO2 + O2 + vitamin K hydroquinone proPT28: synthetic peptide is designated by the following nomenclature: pro indicates the presence of the propeptide sequence, PT indicates prothrombin, the protein on which the peptide sequence is based, and 28 indicates the number of amino acid residues in the peptide Bos taurus ? + vitamin K epoxide + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 77000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
glutamate carboxylase
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
vitamin K
-
 Bos taurus