Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is independent of the branched chain amino acids leucine, valine or isoleucine | Leuconostoc lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by EDTA | Leuconostoc lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is independent of metals | Leuconostoc lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Leuconostoc lactis |
100000 | - |
approximately, gel filtration | Leuconostoc lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leuconostoc lactis | citrate metabolism | ? | - |
? | |
additional information | Leuconostoc lactis NCW1 | citrate metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc lactis | - |
- |
- |
Leuconostoc lactis NCW1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
5333fold | Leuconostoc lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Leuconostoc lactis |
Storage Stability | Organism |
---|---|
-70°C, purified, aliquoted ALD, 30% (v/v) glycerol, 3 weeks, stable | Leuconostoc lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Leuconostoc lactis | (R)-acetoin + CO2 | - |
? | |
(S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Leuconostoc lactis NCW1 | (R)-acetoin + CO2 | - |
? | |
additional information | ALD does not catalyze the direct production of diacetyl from 2-acetolactate | Leuconostoc lactis | ? | - |
? | |
additional information | citrate metabolism | Leuconostoc lactis | ? | - |
? | |
additional information | ALD does not catalyze the direct production of diacetyl from 2-acetolactate | Leuconostoc lactis NCW1 | ? | - |
? | |
additional information | citrate metabolism | Leuconostoc lactis NCW1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, SDS-PAGE | Leuconostoc lactis |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Leuconostoc lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to | Leuconostoc lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis | |
66.67 | - |
(S)-2-Hydroxy-2-methyl-3-oxobutanoate | pH 6 | Leuconostoc lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Leuconostoc lactis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leuconostoc lactis | Mono P chromatofocusing | 4.4 | 4.3 |