Any feedback?
Literature summary for 4.1.1.49 extracted from
- Molecular characterization, enzyme properties and transcriptional regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in a ruminal bacterium, Selenomonas ruminantium (2001), Microbiology, 147, 681-690.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Selenomonas ruminantium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | transcription of pck gene is suppressed by | Selenomonas ruminantium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
ADP | pH 7.0, 37°C | Selenomonas ruminantium | |
| 0.55 | - |
phosphoenolpyruvate | pH 7.0, 37°C | Selenomonas ruminantium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | both Mg2+ and Mn2+ required for maximal activity | Selenomonas ruminantium | |
| Mn2+ | both Mn2+ and Mg2+ required for maximal activity | Selenomonas ruminantium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59570 | - |
deduced from pck operon | Selenomonas ruminantium |
| 59600 | - |
- |
Selenomonas ruminantium |
| 60000 | - |
gel filtration | Selenomonas ruminantium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | Selenomonas ruminantium | - |
ATP + oxaloacetate | - |
r | |
| ADP + phosphoenolpyruvate + CO2 | Selenomonas ruminantium TH1 | - |
ATP + oxaloacetate | - |
r | |
| ATP + oxaloacetate | Selenomonas ruminantium | - |
ADP + phosphoenolpyruvate + CO2 | - |
r | |
| ATP + oxaloacetate | Selenomonas ruminantium TH1 | - |
ADP + phosphoenolpyruvate + CO2 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Selenomonas ruminantium | O83023 | - |
- |
| Selenomonas ruminantium TH1 | O83023 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Selenomonas ruminantium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | - |
Selenomonas ruminantium | ATP + oxaloacetate | - |
r | |
| ADP + phosphoenolpyruvate + CO2 | - |
Selenomonas ruminantium TH1 | ATP + oxaloacetate | - |
r | |
| ATP + oxaloacetate | - |
Selenomonas ruminantium | ADP + phosphoenolpyruvate + CO2 | - |
r | |
| ATP + oxaloacetate | - |
Selenomonas ruminantium TH1 | ADP + phosphoenolpyruvate + CO2 | - |
r | |
| additional information | GDP and IDP are no substrates | Selenomonas ruminantium | ? | - |
? | |
| additional information | GDP and IDP are no substrates | Selenomonas ruminantium TH1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Selenomonas ruminantium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCK | - |
Selenomonas ruminantium |
| phosphoenolpyruvate carboxykinase | - |
Selenomonas ruminantium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Selenomonas ruminantium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | half-maximal activity at pH 6.0 and pH 8.5 | Selenomonas ruminantium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
