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Literature summary for 4.1.1.49 extracted from
- Purification and characterization of phosphoenolpyruvate carboxykinase, a catabolic CO2-fixing enzyme, from Anaerobiospirillum succiniciproducens (1993), J. Gen. Microbiol., 139, 223-228.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Anaerobiospirillum succiniciproducens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
ADP | - |
Anaerobiospirillum succiniciproducens | |
| 0.54 | - |
phosphoenolpyruvate | - |
Anaerobiospirillum succiniciproducens | |
| 1.2 | - |
oxaloacetate | - |
Anaerobiospirillum succiniciproducens | |
| 2.3 | - |
ATP | - |
Anaerobiospirillum succiniciproducens | |
| 2.77 | - |
CO2 | CO2 in form of NaHCO3 | Anaerobiospirillum succiniciproducens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates in presence of Mg2+ | Anaerobiospirillum succiniciproducens | |
| Mg2+ | activates | Anaerobiospirillum succiniciproducens | |
| Mn2+ | activation | Anaerobiospirillum succiniciproducens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Anaerobiospirillum succiniciproducens |
| 58000 | - |
1 * 58000, SDS-PAGE | Anaerobiospirillum succiniciproducens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | Anaerobiospirillum succiniciproducens | catabolic CO2-fixing enzyme, important role in succinate formation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anaerobiospirillum succiniciproducens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Anaerobiospirillum succiniciproducens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Anaerobiospirillum succiniciproducens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | - |
Anaerobiospirillum succiniciproducens | ATP + oxaloacetate | - |
? | |
| ADP + phosphoenolpyruvate + CO2 | catabolic CO2-fixing enzyme, important role in succinate formation | Anaerobiospirillum succiniciproducens | ? | - |
? | |
| additional information | no activity with IDP, GDP, ITP and GTP | Anaerobiospirillum succiniciproducens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Anaerobiospirillum succiniciproducens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.67 | - |
phosphoenolpyruvate | - |
Anaerobiospirillum succiniciproducens | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
complete loss of activity below | Anaerobiospirillum succiniciproducens |
| 5 | 9 | stable in the range | Anaerobiospirillum succiniciproducens |
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