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Literature summary for 4.1.1.39 extracted from
- Distinct properties of Escherichia coli products of plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum (1990), J. Biol. Chem., 265, 18386-18392.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Allochromatium vinosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
D-ribulose 1,5-bisphosphate | mutants rbcL-rbcS, rbcA-rbcD | Allochromatium vinosum |  |
| 0.022 | - |
D-ribulose 1,5-bisphosphate | mutant rbcL-rbcS | Allochromatium vinosum | |
| 0.033 | - |
CO2 | wild-type and mutant rbcA-rbcD | Allochromatium vinosum | |
| 0.072 | - |
CO2 | mutant rbcL-rbcS | Allochromatium vinosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 530000 | - |
non-denaturing gel filtration | Allochromatium vinosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 1,5-bisphosphate + CO2 | Allochromatium vinosum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allochromatium vinosum | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O | D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction | Allochromatium vinosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.11 | - |
expressed in Escherichia coli, plasmid pCKS1, carboxylase | Allochromatium vinosum |
| 6.7 | - |
expressed in Escherichia coli, plasmid pCV23, carboxylase | Allochromatium vinosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 1,5-bisphosphate + CO2 | - |
Allochromatium vinosum | D-3-Phosphoglycerate | - |
? | |
| D-Ribulose 1,5-bisphosphate + CO2 | - |
Allochromatium vinosum | ? | - |
? | |
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