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Literature summary for 4.1.1.31 extracted from

  • Rivoal, J.; Plaxton, W.C.; Turpin, D.H.
    Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzymes (1998), Biochem. J., 331, 201-209.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dihydroxyacetone phosphate activates Chlamydomonas reinhardtii
glutamine activates Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Chlamydomonas reinhardtii
Asp
-
Chlamydomonas reinhardtii
Glu
-
Chlamydomonas reinhardtii
malate
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
sequencing of the N-terminus, peptide mapping and immunological data suggest that the catalytic subunit of the enzyme is not related to the prokaryotic enzyme and is only distantly related to higher plant C4 and C3 enzymes Chlamydomonas reinhardtii
100000
-
4 * 100000, isoenzyme PEPC1, SDS-PAGE Chlamydomonas reinhardtii
400000
-
low-molecular-mass isoenzyme PEPC1, gel filtration Chlamydomonas reinhardtii
650000
-
high-molecular-weight isoenzyme PEPC2, gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphoenolpyruvate + CO2 Chlamydomonas reinhardtii key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae ?
-
?
Phosphoenolpyruvate + CO2 Chlamydomonas reinhardtii CW-15 cc1883 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae ?
-
?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii CW-15 cc1883
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a high-molecular-mass isoform, PEPC2, and a low-molecular-mass isoform, PEPC1 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.11
-
-
Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Chlamydomonas reinhardtii phosphate + oxaloacetate
-
?
phosphoenolpyruvate + CO2
-
Chlamydomonas reinhardtii CW-15 cc1883 phosphate + oxaloacetate
-
?
Phosphoenolpyruvate + CO2 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae Chlamydomonas reinhardtii ?
-
?
Phosphoenolpyruvate + CO2 key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae Chlamydomonas reinhardtii CW-15 cc1883 ?
-
?

Subunits

Subunits Comment Organism
? isoenzyme PEPC2 is a complex between the PEPC catalytic subunit of MW 100000 Da and other immunologically unrelated polypeptides of 50000-700000 Da Chlamydomonas reinhardtii
tetramer 4 * 100000, isoenzyme PEPC1, SDS-PAGE Chlamydomonas reinhardtii