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Literature summary for 3.5.1.75 extracted from
- Urethanase in rat tissues (1992), J. Toxicol. Environ. Health, 37, 37-45.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | slight activation, activation depending on increasing concentrations of Triton X-100 | Rattus norvegicus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong inhibition, 0.00074 mM: 50% inhibition. Complete inhibition fully recovered by addition of Zn2+, but no activity recovered by addition of Mg2+ | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | moderate activity | Rattus norvegicus | 5829 | - |
| lysosome | highest activity, located mainly in the lysosomal fraction | Rattus norvegicus | 5764 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| mitochondrion | moderate activity | Rattus norvegicus | 5739 | - |
| additional information | intracellular distribution | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect, activation by Co2+, Ni2+, Mn2+, Fe2+, Cu2+, Ca2+ | Rattus norvegicus | |
| Zn2+ | probably belongs to category of Zn enzyme | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylcarbamate + H2O | Rattus norvegicus | urethane, potentially carcinogenic, mutagenic and teratogenic to human | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | Rattus norvegicus | urethane, potentially carcinogenic, mutagenic and teratogenic to human | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| ethylcarbamate + H2O | Rattus norvegicus | enzyme may play an important role in detoxification of urethane | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | Rattus norvegicus | enzyme may play an important role in detoxification of urethane | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | highest activity | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| additional information | tissue distribution | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Rattus norvegicus | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Rattus norvegicus | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| ethylcarbamate + H2O | enzyme may play an important role in detoxification of urethane | Rattus norvegicus | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | enzyme may play an important role in detoxification of urethane | Rattus norvegicus | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable at for 1 h | Rattus norvegicus |
| 80 | - |
1 h, complete loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
significantly higher urethanase activity at acidic condition than at neutral condition | Rattus norvegicus |
| 5 | - |
- |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | stable between | Rattus norvegicus |
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