Literature summary for 3.5.1.137 extracted from

Naqvi, T.; Cheesman, M.J.; Williams, M.R.; Campbell, P.M.; Ahmed, S.; Russell, R.J.; Scott, C.; Oakeshott, J.G.
Heterologous expression of the methyl carbamate-degrading hydrolase MCD (2009), J. Biotechnol., 144, 89-95 .

Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(lambdaDE3) cells	Achromobacter sp. WM111

Inhibitors

Inhibitors	Comment	Organism	Structure
acetone	20% residual activity in 25% (v/v) acetone	Achromobacter sp. WM111
acetonitrile	20% residual activity in 14% (v/v) acetonitrile	Achromobacter sp. WM111
Cu2+	complete inhibition at 1 mM	Achromobacter sp. WM111
EDTA	about 40% inhibition at 100 mM	Achromobacter sp. WM111
Fe2+	complete inhibition at 1 mM	Achromobacter sp. WM111
Fe3+	complete inhibition at 1 mM	Achromobacter sp. WM111
methanol	20% residual activity in 25% (v/v) methanol	Achromobacter sp. WM111
Phenanthroline	complete inhibition at 10 mM	Achromobacter sp. WM111
Zn2+	complete inhibition at 1 mM	Achromobacter sp. WM111

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.031	-	Carbaryl	at pH 6.8 and 37°C	Achromobacter sp. WM111
0.167	-	dimethyl umbelliferyl phosphate	at pH 6.8 and 37°C	Achromobacter sp. WM111
0.223	-	1-naphthyl acetate	at pH 6.8 and 37°C	Achromobacter sp. WM111

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	dependent on	Achromobacter sp. WM111

Organism

Organism	UniProt	Comment	Textmining
Achromobacter sp. WM111	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl Sepharose column chromatography and Superdex 200 gel filtration	Achromobacter sp. WM111

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-naphthyl acetate + H2O	-	Achromobacter sp. WM111	1-naphthol + acetate	-	?
carbaryl + H2O	-	Achromobacter sp. WM111	1-naphthol + methylamine + CO2	-	?
dimethyl umbelliferyl phosphate + H2O	-	Achromobacter sp. WM111	umbelliferone + dimethyl phosphate	-	?
additional information	no activity with diethyl umbelliferyl phosphate, chlorpyrifos and parathion	Achromobacter sp. WM111	?	-	-

Subunits

Subunits	Comment	Organism
homodimer	2 * 77000, SDS-PAGE	Achromobacter sp. WM111

Synonyms

Synonyms	Comment	Organism
MCD	-	Achromobacter sp. WM111
methyl carbamate-degrading hydrolase	-	Achromobacter sp. WM111

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	55	incubation at temperatures in excess of 45°C reduce the carbaryl hydrolase activity of the enzyme, with half maximal activity at approximately 55°C. However, incubation at between 30°C and 45°C cause an overall increase in activity	Achromobacter sp. WM111

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00000051	-	dimethyl umbelliferyl phosphate	at pH 6.8 and 37°C	Achromobacter sp. WM111
1.7	-	Carbaryl	at pH 6.8 and 37°C	Achromobacter sp. WM111
52.7	-	1-naphthyl acetate	at pH 6.8 and 37°C	Achromobacter sp. WM111

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.1	-	dimethyl umbelliferyl phosphate	at pH 6.8 and 37°C	Achromobacter sp. WM111
54	-	Carbaryl	at pH 6.8 and 37°C	Achromobacter sp. WM111
240	-	1-naphthyl acetate	at pH 6.8 and 37°C	Achromobacter sp. WM111

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Release 2023.1 (January 2023)