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Literature summary for 3.5.1.137 extracted from

  • Derbyshire, M.; Karns, J.; Kearney, P.; Nelson, J.
    Purification and characterization of an N-methylcarbamate pesticide hydrolyzing enzyme (1987), J. Agric. Food Chem., 35, 871-877 .
No PubMed abstract available
Search for more literature for 3.5.1.137

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 Carbaryl at pH 8.3 and 25°C Achromobacter sp. WM111
0.056
-
 carbofuran at pH 8.3 and 25°C Achromobacter sp. WM111
2.8
-
 Aldicarb at pH 8.3 and 25°C Achromobacter sp. WM111

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 gel filtration Achromobacter sp. WM111

Organism

Organism UniProt Comment Textmining
Achromobacter sp. WM111
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate precipitation, ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Biogel A0.5m gel filtration Achromobacter sp. WM111

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
 crude extract, with 4-nitrophenyl acetate as substrate, at pH 8.3 and 25°C Achromobacter sp. WM111
0.182
-
 after 7.3fold purification, with 4-nitrophenyl acetate as substrate, at pH 8.3 and 25°C Achromobacter sp. WM111

Storage Stability

Storage Stability Organism
0°C, purified enzyme, 48 h, no loss of activity Achromobacter sp. WM111

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Achromobacter sp. WM111 4-nitrophenol + acetate
-
 ?
aldicarb + H2O i.e. 2-methyl-2-(methylthio)propionaldehyde O-(N-methylcarbamoyl)oxime Achromobacter sp. WM111 ? + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Achromobacter sp. WM111 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O i.e. 2,3-dihydro-2,2-dimethyl-7-benzofuranyl N-methylcarbamate Achromobacter sp. WM111 2,3-dihydro-2,2-dimethyl-7-benzofuranol + methylamine + CO2
-
 ?
additional information the enzyme is unable to hydrolyze parathion (O,O-diethyl O-(4-nitrophenyl) phosphorothioate), S-ethyl dipropylthiocarbamate, or chlorpropham (isopropyl 3-chlorocarbanilate) Achromobacter sp. WM111 ?
-
-

Synonyms

Synonyms Comment Organism
carbofuran hydrolase
-
 Achromobacter sp. WM111

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 53
-
 Achromobacter sp. WM111

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10.5
-
 Achromobacter sp. WM111

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme does not require any cofactor Achromobacter sp. WM111