Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chloromercuribenzoate | 21% residual activity at 1 mM | Aspergillus niger | |
Ag+ | complete inhibition at 1 mM | Aspergillus niger | |
Cu2+ | 91% residual activity at 1 mM | Aspergillus niger | |
diisopropylfluorophosphate | 33.6% residual activity at 1 mM | Aspergillus niger | |
Hg2+ | complete inhibition at 1 mM | Aspergillus niger | |
iodoacetic acid | 13% residual activity at 1 mM | Aspergillus niger | |
Mg2+ | 93% residual activity at 1 mM | Aspergillus niger | |
Mn2+ | 96% residual activity at 1 mM | Aspergillus niger | |
additional information | not inhibited by EDTA and phenanthroline | Aspergillus niger | |
phenylmethylsulfonyl fluoride | 24.6% residual activity at 1 mM | Aspergillus niger | |
Zn2+ | 95% residual activity at 1 mM | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger | |
0.036 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
0.039 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
0.041 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
0.053 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
0.067 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
0.093 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
0.387 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
0.551 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
0.587 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
1.038 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger PY168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate precipitation, ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl 200 gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.7 | - |
cell extract, at pH 7.0 and 30°C | Aspergillus niger |
968 | - |
after 262fold purification, at pH 7.0 and 30°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | 73% activity compared to carbaryl | Aspergillus niger | 1-naphthol + acetate | - |
? | |
1-naphthyl acetate + H2O | 73% activity compared to carbaryl | Aspergillus niger PY168 | 1-naphthol + acetate | - |
? | |
3,5-dimethylphenyl N-methylcarbamate + H2O | 91% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
3,5-dimethylphenyl N-methylcarbamate + H2O | 91% activity compared to carbaryl | Aspergillus niger PY168 | ? + methylamine + CO2 | - |
? | |
4-nitrophenyl acetate + H2O | 77% activity compared to carbaryl | Aspergillus niger | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 77% activity compared to carbaryl | Aspergillus niger PY168 | 4-nitrophenol + acetate | - |
? | |
aldicarb + H2O | 18% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
carbaryl + H2O | preferred substrate, 100% activity | Aspergillus niger | 1-naphthol + methylamine + CO2 | - |
? | |
carbaryl + H2O | preferred substrate, 100% activity | Aspergillus niger PY168 | 1-naphthol + methylamine + CO2 | - |
? | |
carbofuran + H2O | 83% activity compared to carbaryl | Aspergillus niger | carbofuran phenol + methylamine + CO2 | - |
? | |
fenobucarb + H2O | 32% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
isoprocarb + H2O | 43% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
metolcarb + H2O | 87% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
additional information | hydrolase activity is undetectable with chloropropham, S-ethyl dipropylthiocarbamate, and parathion | Aspergillus niger | ? | - |
- |
|
additional information | hydrolase activity is undetectable with chloropropham, S-ethyl dipropylthiocarbamate, and parathion | Aspergillus niger PY168 | ? | - |
- |
|
propoxur + H2O | 54% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? | |
xylylcarb + H2O | 93% activity compared to carbaryl | Aspergillus niger | ? + methylamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
carbaryl hydrolase | - |
Aspergillus niger |
cehA | - |
Aspergillus niger |
cfdJ | - |
Aspergillus niger |
mcbA | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | more than 50% activity between 30 and 55 C. The enzyme has 56% of its full activity at 55 C and is completely inactivated at 65 C | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
548 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger | |
613 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
670 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
717 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
906 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
1070 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
1282 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
1369 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
1581 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
1633 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
1724 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | more than 75% activity between pH 5.5 and 9.0 | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme remains stable at pH 6.0 and 8.0 with more than 90% activity remaining after 1 h | Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing | - |
4.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
530 | - |
Aldicarb | at pH 7.0 and 30°C | Aspergillus niger | |
1040 | - |
fenobucarb | at pH 7.0 and 30°C | Aspergillus niger | |
1210 | - |
isoprocarb | at pH 7.0 and 30°C | Aspergillus niger | |
1850 | - |
propoxur | at pH 7.0 and 30°C | Aspergillus niger | |
17000 | - |
3,5-dimethylphenyl N-methylcarbamate | at pH 7.0 and 30°C | Aspergillus niger | |
17100 | - |
1-naphthyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
19300 | - |
carbofuran | at pH 7.0 and 30°C | Aspergillus niger | |
29700 | - |
4-nitrophenyl acetate | at pH 7.0 and 30°C | Aspergillus niger | |
33400 | - |
metolcarb | at pH 7.0 and 30°C | Aspergillus niger | |
41800 | - |
xylylcarb | at pH 7.0 and 30°C | Aspergillus niger | |
144000 | - |
Carbaryl | at pH 7.0 and 30°C | Aspergillus niger |