Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 110% activity at 1 mM | Arthrobacter sp. RC100 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 90% residual activity at 1 mM | Arthrobacter sp. RC100 | |
diisopropylfluorophosphate | 37% residual activity at 1 mM | Arthrobacter sp. RC100 | |
Hg2+ | 20% residual activity at 1 mM | Arthrobacter sp. RC100 | |
additional information | not inhibited by EDTA, N-ethylmaleimide and iodoacetamide | Arthrobacter sp. RC100 | |
paraoxon | less than 1% residual activity at 1 mM | Arthrobacter sp. RC100 | |
phenylmethylsulfonyl fluoride | less than 4% residual activity at 1 mM | Arthrobacter sp. RC100 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Arthrobacter sp. RC100 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an N-methyl carbamate ester + H2O | Arthrobacter sp. RC100 | - |
an alcohol + methylamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. RC100 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate treatment, ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Arthrobacter sp. RC100 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.043 | - |
crude extract, at pH 7.0 and 30°C | Arthrobacter sp. RC100 |
0.388 | - |
after 9.1fold purification, at pH 7.0 and 30°C | Arthrobacter sp. RC100 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | 51% activity compared to carbaryl | Arthrobacter sp. RC100 | 1-naphthol + acetate | - |
? | |
3,5-dimethylphenyl N-methylcarbamate + H2O | 43% activity compared to carbaryl | Arthrobacter sp. RC100 | ? + methylamine + CO2 | - |
? | |
4-nitrophenyl acetate + H2O | 80% activity compared to carbaryl | Arthrobacter sp. RC100 | 4-nitrophenol + acetate | - |
? | |
an N-methyl carbamate ester + H2O | - |
Arthrobacter sp. RC100 | an alcohol + methylamine + CO2 | - |
? | |
carbaryl + H2O | 100% activity | Arthrobacter sp. RC100 | 1-naphthol + methylamine + CO2 | - |
? | |
metolcarb + H2O | 304% activity compared to carbaryl | Arthrobacter sp. RC100 | ? + methylamine + CO2 | - |
? | |
additional information | the enzyme is not able to hydrolyze chloropropham, fenobucarb, propoxur, and isoprocarb | Arthrobacter sp. RC100 | ? | - |
- |
|
xylylcarb + H2O | 66% activity compared to carbaryl | Arthrobacter sp. RC100 | ? + methylamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 51000, SDS-PAGE | Arthrobacter sp. RC100 |
Synonyms | Comment | Organism |
---|---|---|
carbaryl hydrolase | - |
Arthrobacter sp. RC100 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Arthrobacter sp. RC100 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | 40% of the initial activity is retained after 60 min at 60°C. The enzyme is completely inactivated by heating for 60 min at 70°C | Arthrobacter sp. RC100 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Arthrobacter sp. RC100 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme retains over 80% of its maximum activity between pH 6.0 and 10.0 | Arthrobacter sp. RC100 |