Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | inhibits activity | Arabidopsis thaliana | |
molybdate | - |
Arabidopsis thaliana | |
vanadate | - |
Arabidopsis thaliana | |
Zn2+ | inhibits activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
phosphoenolpyruvate | pH 5.6, 25°C | Arabidopsis thaliana | |
0.178 | - |
phosphoenolpyruvate | pH 5.6, 25°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enhances activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Arabidopsis thaliana |
60000 | - |
SDS-PAGE | Arabidopsis thaliana |
130000 | - |
gel filtration, native molecular mass | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Arabidopsis thaliana |
glycoprotein | differential glycosylation influences the subcellular targeting and substrate selectivity of AtPAP26 | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | relative activity: 43% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 1-naphthol + phosphate | - |
? | |
1-naphthyl phosphate + H2O | relative activity: 53% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | relative activity: 116% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 2-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | relative activity: 118% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 2-naphthol + phosphate | - |
? | |
3-phosphoglycerate + H2O | relative activity: 38% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | glycerate + phosphate | - |
? | |
3-phosphoglycerate + H2O | relative activity: 67% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | relative activity: 101% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | relative activity: 83% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
6-phospho-D-gluconate + H2O | relative activity: 67% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | gluconate + phosphate | - |
? | |
6-phospho-D-gluconate + H2O | relative activity: 69% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | gluconate + phosphate | - |
? | |
ADP + H2O | relative activity: 68% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | AMP + phosphate | - |
? | |
ADP + H2O | relative activity: 77% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | AMP + phosphate | - |
? | |
ATP + H2O | relative activity: 52% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | ADP + phosphate | - |
? | |
ATP + H2O | relative activity: 81% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | ADP + phosphate | - |
? | |
D-ribose 3-phosphate + H2O | relative activity: 25% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | D-ribose + phosphate | - |
? | |
D-ribose 3-phosphate + H2O | relative activity: 41% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | D-ribose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | relative activity: 50% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | D-ribose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | relative activity: 9% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | D-ribose + phosphate | - |
? | |
dAMP + H2O | relative activity: 12% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | deoxyadenosine + phosphate | - |
? | |
dAMP + H2O | relative activity: 19% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | deoxyadenosine + phosphate | - |
? | |
glycerol 3-phosphate + H2O | relative activity: 44% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
glycerol 3-phosphate + H2O | relative activity: 59% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
GTP + H2O | relative activity: 29% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | GDP + phosphate | - |
? | |
GTP + H2O | relative activity: 45% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | GDP + phosphate | - |
? | |
L-phosphoserine + H2O | relative activity: 14% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | L-serine + phosphate | - |
? | |
L-phosphoserine + H2O | relative activity: 26% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | L-serine + phosphate | - |
? | |
L-phosphothreonine + H2O | relative activity: 29% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | L-threonine + phosphate | - |
? | |
L-phosphothreonine + H2O | relative activity: 49% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | L-threonine + phosphate | - |
? | |
Na diphosphate + H2O | relative activity: 41% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | ? | - |
? | |
Na diphosphate + H2O | relative activity: 63% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | ? | - |
? | |
phenyl phosphate + H2O | relative activity: 96% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | phenol + phosphate | - |
? | |
phenyl phosphate + H2O | relative activity: 98% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | phenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | relative activity: 100% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | pyruvate + phosphate | - |
? | |
tyrosine phosphate + H2O | relative activity: 60% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | tyrosine + phosphate | - |
? | |
tyrosine phosphate + H2O | relative activity: 71% compared to phosphoenolpyruvate as substrate | Arabidopsis thaliana | tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration, 2 * 60000 | Arabidopsis thaliana |
monomer | 1 * 55000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase | - |
Arabidopsis thaliana |
AtPAP12 | - |
Arabidopsis thaliana |
AtPAP26 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Arabidopsis thaliana |
5.6 | - |
assay at | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | significant activity | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | AtPAP26 is markedly up-regulated in response to phosphate deprivation | up |
General Information | Comment | Organism |
---|---|---|
malfunction | analysis of atpap12 and atpap26 T-DNA mutants verifies that AtPAP12 and AtPAP26 account for most of the secreted acid phosphatase activity of phosphate-deficient wild-type seedlings | Arabidopsis thaliana |
physiological function | AtPAP26 is dual targeted during phosphate stress because it is also the principal intracellular (vacuolar) PAP up-regulated by phosphate-deficient Arabidopsis | Arabidopsis thaliana |