Cloned (Comment) | Organism |
---|---|
gene aphA, overexpression in strain DH5alpha | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | IC50 is 0.0020 mM | Escherichia coli | |
dipicolinic acid | IC50 is 0.0025 mM | Escherichia coli | |
EDTA | IC50 is 0.0004 mM | Escherichia coli | |
EGTA | IC50 is 0.0025 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Escherichia coli | |
0.0013 | - |
3'-dCMP | pH 6.0, 37°C, recombinant AphA | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AE22 | gene aphA | - |
Escherichia coli MG1655 | P0AE22 | gene aphA | - |
Purification (Comment) | Organism |
---|---|
recombinant AphA from strain DH5alpha | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | modelling of substrate binding in the active site | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | - |
Escherichia coli | adenosine + phosphate | - |
? | |
3'-AMP + H2O | - |
Escherichia coli MG1655 | adenosine + phosphate | - |
? | |
3'-CMP + H2O | - |
Escherichia coli | cytidine + phosphate | - |
? | |
3'-dAMP + H2O | - |
Escherichia coli | 3'-deoxyadenosine + phosphate | - |
? | |
3'-dCMP + H2O | best substrate | Escherichia coli | deoxycytidine + phosphate | - |
? | |
3'-dGMP + H2O | - |
Escherichia coli | deoxyguanosine + phosphate | - |
? | |
3'-dUMP + H2O | - |
Escherichia coli | deoxyuridine + phosphate | - |
? | |
3'-GMP + H2O | - |
Escherichia coli | guanosine + phosphate | - |
? | |
3'-UMP + H2O | - |
Escherichia coli | uridine + phosphate | - |
? | |
3-phosphoglycerate + H2O | low activity | Escherichia coli | glycerate + phosphate | - |
? | |
3-phosphoglycerate + H2O | low activity | Escherichia coli MG1655 | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? | |
5'-AMP + H2O | - |
Escherichia coli | adenosine + phosphate | - |
? | |
5'-AMP + H2O | - |
Escherichia coli MG1655 | adenosine + phosphate | - |
? | |
5'-CMP + H2O | - |
Escherichia coli | cytidine + phosphate | - |
? | |
5'-dAMP + H2O | - |
Escherichia coli | deoxyadenosine + phosphate | - |
? | |
5'-dCMP + H2O | - |
Escherichia coli | deoxycytidine + phosphate | - |
? | |
5'-dGMP + H2O | - |
Escherichia coli | deoxyguanosine + phosphate | - |
? | |
5'-dUMP + H2O | - |
Escherichia coli | deoxyuridine + phosphate | - |
? | |
5'-GMP + H2O | - |
Escherichia coli | guanosine + phosphate | - |
? | |
5'-UMP + H2O | - |
Escherichia coli | uridine + phosphate | - |
? | |
ADP + H2O | - |
Escherichia coli | AMP + phosphate | - |
? | |
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | low activity | Escherichia coli | D-glucose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | low activity | Escherichia coli | D-ribose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | low activity | Escherichia coli MG1655 | D-ribose + phosphate | - |
? | |
additional information | substrate specificity of AphA, overview | Escherichia coli | ? | - |
? | |
additional information | substrate specificity of AphA, overview | Escherichia coli MG1655 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AphA | - |
Escherichia coli |
More | the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
101 | - |
3'-dCMP | pH 6.0, 37°C, recombinant AphA | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0004 | - |
IC50 is 0.0004 mM | Escherichia coli | EDTA | |
0.002 | - |
IC50 is 0.0020 mM | Escherichia coli | 1,10-phenanthroline | |
0.0025 | - |
IC50 is 0.0025 mM | Escherichia coli | EGTA | |
0.0025 | - |
IC50 is 0.0025 mM | Escherichia coli | dipicolinic acid |