Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | partial cleavage results in 1.7fold enzyme activation | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expression in Spodopetra frugiperda Sf9 cells using the baculovirus infection system, the recombinant enzyme is secreted from the cell culture | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pepsin | partial cleavage results in enzyme inhibition | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | two iron ions per enzyme molecule | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
1 * 35000-37000 + 1 * 67000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | the enzyme is an iron-binding protein required for maternal/fetal iron transport | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant enzyme | Sus scrofa |
Purification (Comment) | Organism |
---|---|
recombinant secreted enzyme 155fold from Sf9 insect cell culture expressing the enzyme by ion exchange, adsorption, and affinity chromatography, and gel filtration | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
uterine endometrium | - |
Sus scrofa | - |
uterus | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
recombinant enzyme in cell culture medium | Sus scrofa |
233 | - |
purified recombinant enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Sus scrofa | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme is an iron-binding protein required for maternal/fetal iron transport | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 35000-37000 + 1 * 67000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Sus scrofa |
purple acid phosphatase | - |
Sus scrofa |
Uf | - |
Sus scrofa |
uteroferrin | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Sus scrofa |