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Literature summary for 3.1.3.2 extracted from

  • Filburn, C.R.
    Acid phosphatase isoenzymes of Xenopus laevis tadpole tails. Separation and partial characterization (1973), Arch. Biochem. Biophys., 159, 683-693.
No PubMed abstract available
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activates isoenzyme AP-4 Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 1 mM, strong inhibition of the 4 isoenzymes Xenopus laevis
EDTA
-
 Xenopus laevis
F-
-
 Xenopus laevis
Hg2+ strong inhibition of the isoenzymes AP-2, AP-3 and AP-4, less inhibitory to isoenzyme AP-1 Xenopus laevis
Mn2+ 10 mM, slight inhibition of the 4 isoenzymes Xenopus laevis
NaCl
-
 Xenopus laevis
phosphate
-
 Xenopus laevis
Tartrate D-(+)-tartrate Xenopus laevis
Triton X-100 inhibits isoenzyme AP-1 Xenopus laevis
Urea
-
 Xenopus laevis
Zn2+ 1 mM, strong inhibition of isoenzyme AP-3 and AP-4, weak inhibition of isoenzyme AP-1 and AP-2 Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18700
-
 isoenzyme AP-4, gel filtration Xenopus laevis
40900
-
 isoenzyme AP-3 Xenopus laevis
109000
-
 isoenzyme AP-2, gel filtration Xenopus laevis
200000
-
 above, isoenzyme AP-1, gel filtration Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tail 4 isoenzymes: AP-1, AP-2, AP-3 and AP-4 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-glycerate phosphate + H2O isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4 Xenopus laevis glycerate + phosphate
-
 ?
1-naphthyl phosphate + H2O
-
 Xenopus laevis 1-naphthol + phosphate
-
 ?
2,3-diphosphoglycerate + H2O isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4 Xenopus laevis ?
-
 ?
2-glycerophosphate + H2O isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis glycerol + phosphate
-
 ?
5'-AMP + H2O isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 Xenopus laevis adenosine + phosphate
-
 ?
5'-CMP + H2O isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 Xenopus laevis cytidine + phosphate
-
 ?
5'-GMP + H2O isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 Xenopus laevis guanosine + phosphate
-
 ?
5'-IMP + H2O isoenzyme AP1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis ?
-
 ?
5'-UMP + H2O isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 Xenopus laevis uridine + phosphate
-
 ?
ADP + H2O isoenzyme AP-1 and AP-3, very low activity with isoenzyme AP-4, no activity with isoenzyme AP-2 Xenopus laevis AMP + phosphate
-
 ?
alpha-glycerophosphate + H2O isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis glycerol + phosphate
-
 ?
ATP + H2O
-
 Xenopus laevis ADP + phosphate
-
 ?
bis(p-nitrophenyl) phosphate + H2O low activity with the four isoenzymes Xenopus laevis ?
-
 ?
CTP + H2O
-
 Xenopus laevis CDP + phosphate
-
 ?
D-fructose 1,6-diphosphate + H2O only weak activity with isoenzyme AP-3 and AP-4, higher activity with isoenzyme AP-1 and AP-2 Xenopus laevis ?
-
 ?
D-ribose 5-phosphate + H2O isoenzyme AP-1 and AP-2, weak activity with isoenzyme AP-4, no activity with isoenzyme AP-3 Xenopus laevis D-ribose + phosphate
-
 ?
dihydroxyacetone phosphate + H2O isoenzyme AP-1 and AP-2, weak activity with isoenzyme AP-4, no activity with isoenzyme AP-3 Xenopus laevis dihydroxyacetone + phosphate
-
 ?
galactose 6-phosphate + H2O weak activity with isoenzyme AP-1, AP-2 and AP-4, no activity with isoenzyme AP-3 Xenopus laevis galactose + phosphate
-
 ?
Glucose 6-phosphate + H2O isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis Glucose + phosphate
-
 ?
additional information isoenzyme AP-1 and AP-2 hydrolyze nearly all substrates tested, isoenzyme AP-3 hydrolyzes significantly only p-nitrophenyl phosphate, alpha-naphthyl phosphate, phosphoenolpyruvate and nucleoside diphosphate and nucleoside triphosphates, isoenzyme AP-4 hydrolyzes significantly only p-nitrophenyl phosphate and riboflavin 5'-phosphate Xenopus laevis ?
-
 ?
O-phosphoethanolamine + H2O very low activity with isoenzyme AP-1, AP-2, and AP-4, no activity with isoenzyme AP-3 Xenopus laevis ethanolamine + phosphate
-
 ?
O-phosphoserine + H2O very low activity with isoenzyme AP-1, AP-2, and AP-4, no activity with isoenzyme AP-3 Xenopus laevis Ser + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Xenopus laevis p-nitrophenol + phosphate
-
 ?
phosphocholine + H2O very low activity with isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis choline + phosphate
-
 ?
phosphopyruvate + H2O isoenzyme AP-1, AP-2 and AP-3, no activity with isoenzyme AP-4 Xenopus laevis pyruvate + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 Xenopus laevis pyridoxal + phosphate
-
 ?
riboflavin 5'-phosphate + H2O isoenzyme AP-1, AP-2 and AP-4, very low activity with isoenzyme AP-3 Xenopus laevis riboflavin + phosphate
-
 ?
UDP + H2O isoenzyme AP-1 and AP-3, no activity with isoenzyme AP-2 and AP-4 Xenopus laevis UDP + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5 isoenzyme AP-1, AP2, and AP-3 Xenopus laevis
5.5 6.5 isoenzyme AP-4 Xenopus laevis