Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | activates isoenzyme AP-4 | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 1 mM, strong inhibition of the 4 isoenzymes | Xenopus laevis | |
EDTA | - |
Xenopus laevis | |
F- | - |
Xenopus laevis | |
Hg2+ | strong inhibition of the isoenzymes AP-2, AP-3 and AP-4, less inhibitory to isoenzyme AP-1 | Xenopus laevis | |
Mn2+ | 10 mM, slight inhibition of the 4 isoenzymes | Xenopus laevis | |
NaCl | - |
Xenopus laevis | |
phosphate | - |
Xenopus laevis | |
Tartrate | D-(+)-tartrate | Xenopus laevis | |
Triton X-100 | inhibits isoenzyme AP-1 | Xenopus laevis | |
Urea | - |
Xenopus laevis | |
Zn2+ | 1 mM, strong inhibition of isoenzyme AP-3 and AP-4, weak inhibition of isoenzyme AP-1 and AP-2 | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18700 | - |
isoenzyme AP-4, gel filtration | Xenopus laevis |
40900 | - |
isoenzyme AP-3 | Xenopus laevis |
109000 | - |
isoenzyme AP-2, gel filtration | Xenopus laevis |
200000 | - |
above, isoenzyme AP-1, gel filtration | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tail | 4 isoenzymes: AP-1, AP-2, AP-3 and AP-4 | Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-glycerate phosphate + H2O | isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4 | Xenopus laevis | glycerate + phosphate | - |
? | |
1-naphthyl phosphate + H2O | - |
Xenopus laevis | 1-naphthol + phosphate | - |
? | |
2,3-diphosphoglycerate + H2O | isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4 | Xenopus laevis | ? | - |
? | |
2-glycerophosphate + H2O | isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | glycerol + phosphate | - |
? | |
5'-AMP + H2O | isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | adenosine + phosphate | - |
? | |
5'-CMP + H2O | isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | cytidine + phosphate | - |
? | |
5'-GMP + H2O | isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | guanosine + phosphate | - |
? | |
5'-IMP + H2O | isoenzyme AP1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | ? | - |
? | |
5'-UMP + H2O | isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | uridine + phosphate | - |
? | |
ADP + H2O | isoenzyme AP-1 and AP-3, very low activity with isoenzyme AP-4, no activity with isoenzyme AP-2 | Xenopus laevis | AMP + phosphate | - |
? | |
alpha-glycerophosphate + H2O | isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | glycerol + phosphate | - |
? | |
ATP + H2O | - |
Xenopus laevis | ADP + phosphate | - |
? | |
bis(p-nitrophenyl) phosphate + H2O | low activity with the four isoenzymes | Xenopus laevis | ? | - |
? | |
CTP + H2O | - |
Xenopus laevis | CDP + phosphate | - |
? | |
D-fructose 1,6-diphosphate + H2O | only weak activity with isoenzyme AP-3 and AP-4, higher activity with isoenzyme AP-1 and AP-2 | Xenopus laevis | ? | - |
? | |
D-ribose 5-phosphate + H2O | isoenzyme AP-1 and AP-2, weak activity with isoenzyme AP-4, no activity with isoenzyme AP-3 | Xenopus laevis | D-ribose + phosphate | - |
? | |
dihydroxyacetone phosphate + H2O | isoenzyme AP-1 and AP-2, weak activity with isoenzyme AP-4, no activity with isoenzyme AP-3 | Xenopus laevis | dihydroxyacetone + phosphate | - |
? | |
galactose 6-phosphate + H2O | weak activity with isoenzyme AP-1, AP-2 and AP-4, no activity with isoenzyme AP-3 | Xenopus laevis | galactose + phosphate | - |
? | |
Glucose 6-phosphate + H2O | isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | Glucose + phosphate | - |
? | |
additional information | isoenzyme AP-1 and AP-2 hydrolyze nearly all substrates tested, isoenzyme AP-3 hydrolyzes significantly only p-nitrophenyl phosphate, alpha-naphthyl phosphate, phosphoenolpyruvate and nucleoside diphosphate and nucleoside triphosphates, isoenzyme AP-4 hydrolyzes significantly only p-nitrophenyl phosphate and riboflavin 5'-phosphate | Xenopus laevis | ? | - |
? | |
O-phosphoethanolamine + H2O | very low activity with isoenzyme AP-1, AP-2, and AP-4, no activity with isoenzyme AP-3 | Xenopus laevis | ethanolamine + phosphate | - |
? | |
O-phosphoserine + H2O | very low activity with isoenzyme AP-1, AP-2, and AP-4, no activity with isoenzyme AP-3 | Xenopus laevis | Ser + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Xenopus laevis | p-nitrophenol + phosphate | - |
? | |
phosphocholine + H2O | very low activity with isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | choline + phosphate | - |
? | |
phosphopyruvate + H2O | isoenzyme AP-1, AP-2 and AP-3, no activity with isoenzyme AP-4 | Xenopus laevis | pyruvate + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4 | Xenopus laevis | pyridoxal + phosphate | - |
? | |
riboflavin 5'-phosphate + H2O | isoenzyme AP-1, AP-2 and AP-4, very low activity with isoenzyme AP-3 | Xenopus laevis | riboflavin + phosphate | - |
? | |
UDP + H2O | isoenzyme AP-1 and AP-3, no activity with isoenzyme AP-2 and AP-4 | Xenopus laevis | UDP + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | isoenzyme AP-1, AP2, and AP-3 | Xenopus laevis |
5.5 | 6.5 | isoenzyme AP-4 | Xenopus laevis |