Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroyxy-5-nitrobenzyl bromide | - |
Aspergillus niger | |
arsenate | - |
Aspergillus niger | |
Borate | - |
Aspergillus niger | |
Bromosuccinimide | - |
Aspergillus niger | |
F- | - |
Aspergillus niger | |
Fe3+ | 8 mM, 45% inhibition | Aspergillus niger | |
molybdate | - |
Aspergillus niger | |
phosphate | - |
Aspergillus niger | |
Tartrate | inhibition by L(+)-tartrate, D(-) tartrate has no effect | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
x * 89000, SDS-PAGE | Aspergillus niger |
310000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 29% carbohydrate consisting of glucosamine, mannose and galactose | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
-15°C, pH 4.6, 0.05 M citrate buffer, stable for at least 3 months | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | 86% of the activity with p-nitrophenyl phosphate | Aspergillus niger | 1-naphthol + phosphate | - |
? | |
2-glycerophosphate + H2O | 72% of the activity with p-nitrophenyl phosphate | Aspergillus niger | glycerol + phosphate | - |
? | |
5'-ADP + H2O | 14% of the activity with p-nitrophenyl phosphate | Aspergillus niger | AMP + phosphate | - |
? | |
5'-AMP + H2O | 105% of the activity with p-nitrophenyl phosphate | Aspergillus niger | adenosine + phosphate | - |
? | |
5'-CMP + H2O | 97% of the activity with p-nitrophenyl phosphate | Aspergillus niger | cytidine + phosphate | - |
? | |
5'-GMP + H2O | 33% of the activity with p-nitrophenyl phosphate | Aspergillus niger | guanosine + phosphate | - |
? | |
6-phosphogluconate + H2O | 17% of the activity with p-nitrophenyl phosphate | Aspergillus niger | gluconate + phosphate | - |
? | |
alpha-glycerophosphate + H2O | 38% of the activity with p-nitrophenyl phosphate | Aspergillus niger | glycerol + phosphate | - |
? | |
bis(p-nitrophenyl) phosphate + H2O | 2% of the activity with p-nitrophenyl phosphate | Aspergillus niger | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | 4% of the activity with p-nitrophenyl phosphate | Aspergillus niger | ? | - |
? | |
D-fructose 6-phosphate + H2O | 32% of the activity with p-nitrophenyl phosphate | Aspergillus niger | D-fructose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | 36% of the activity with p-nitrophenyl phosphate | Aspergillus niger | D-ribose + phosphate | - |
? | |
glucose 1-phosphate + H2O | 45% of the activity with p-nitrophenyl phosphate | Aspergillus niger | glucose + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Aspergillus niger | p-nitrophenol + phosphate | - |
? | |
phosphopyruvate + H2O | 40% of the activity with p-nitrophenyl phosphate | Aspergillus niger | pyruvate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89000, SDS-PAGE | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis of p-nitrophenyl phosphate | Aspergillus niger |