Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
1 * 32500, SDS-PAGE | Bos taurus |
34600 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the charge heterogeneity of isoenzymes might be a result of varying degrees of glycosylation | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | cortical bone matrix | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.15 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | at 12.9% of the activity with p-nitrophenyl phosphate | Bos taurus | AMP + phosphate | - |
? | |
ATP + H2O | at 18.0% of the activity with p-nitrophenyl phosphate | Bos taurus | ADP + phosphate | - |
? | |
CDP + H2O | at 6.6% of the activity with p-nitrophenyl phosphate | Bos taurus | CMP + phosphate | - |
? | |
GDP + H2O | at 18.5% of the activity with p-nitrophenyl phosphate | Bos taurus | GMP + phosphate | - |
? | |
GTP + H2O | at 15.3% of the activity with p-nitrophenyl phosphate | Bos taurus | GDP + phosphate | - |
? | |
O-phospho-L-Tyr + H2O | at 8.2% of the activity with p-nitrophenyl phosphate | Bos taurus | Tyr + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Bos taurus | p-nitrophenol + phosphate | - |
? | |
phosphotyrosyl histone + H2O | 4.2% of the activity with p-nitrophenyl phosphate | Bos taurus | histone + phosphate | - |
? | |
TDP + H2O | at 10.6% of the activity with p-nitrophenyl phosphate | Bos taurus | TMP + phosphate | - |
? | |
TTP + H2O | at 11.3% of the activity with p-nitrophenyl phosphate | Bos taurus | TDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32500, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
hydrolysis of p-nitrophenyl phosphate | Bos taurus |
7 | - |
hydrolysis of nucleoside triphosphate, nucleoside diphosphate, phosphotyrosine and phosphoryltyrosyl histone | Bos taurus |